Document Detail

Magnetite/CdTe magnetic-fluorescent composite nanosystem for magnetic separation and bio-imaging.
MedLine Citation:
PMID:  21464526     Owner:  NLM     Status:  In-Data-Review    
A new synthesis protocol is described to obtain a CdTe decorated magnetite bifunctional nanosystem via dodecylamine (DDA) as cross linker. High resolution transmission electron microscopy (HRTEM), energy-dispersive x-ray spectroscopy (EDAX), vibrating sample magnetometry (VSM), Fourier transform infrared spectroscopy (FTIR), diffuse reflectance spectroscopy (DRS) and fluorescence microscopy are used to characterize the constitution, size, composition and physical properties of these superparamagnetic-fluorescent nanoparticles. These CdTe decorated magnetite nanoparticles were then functionalized with anti-epidermal growth factor receptor (EGFR) antibody to specifically target cells expressing this receptor. The EGFR is a transmembrane glycoprotein and is expressed on tumor cells from different tissue origins including human leukemic cell line Molt-4 cells. The magnetite-CdTe composite nanosystem is shown to perform excellently for specific selection, magnetic separation and fluorescent detection of EGFR positive Molt-4 cells from a mixed population. Flow cytometry and confocal laser scanning microscopy results show that this composite nanosystem has great potential in antibody functionalized magnetic separation and imaging of cells using cell surface receptor antibody.
Anup Kale; Sonia Kale; Prasad Yadav; Haribhau Gholap; Renu Pasricha; J P Jog; Benoit Lefez; Béatrice Hannoyer; Padma Shastry; Satishchandra Ogale
Related Documents :
21347396 - Rnai-mediated knock-down of arylamine n-acetyltransferase-1 expression induces e-cadher...
21237296 - 2d-dige analysis of phospho-enriched fractions from dasatinib-treated melanoma cell lines.
25303116 - Chromatin dynamics in the regulation of cell fate allocation during early embryogenesis.
25434636 - Invasion of uterine cervical squamous cell carcinoma cells is facilitated by locoregion...
9618406 - Role of extracellular thiols in accumulation and distribution of inorganic mercury in r...
22246326 - Multi-isotope imaging mass spectrometry quantifies stem cell division and metabolism.
Publication Detail:
Type:  Journal Article     Date:  2011-04-04
Journal Detail:
Title:  Nanotechnology     Volume:  22     ISSN:  1361-6528     ISO Abbreviation:  Nanotechnology     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-04-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101241272     Medline TA:  Nanotechnology     Country:  England    
Other Details:
Languages:  eng     Pagination:  225101     Citation Subset:  IM    
National Chemical Laboratory, Council of Scientific and Industrial Research, Pune 411008, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  High-throughput optical quality control of lipid multilayers fabricated by dip-pen nanolithography.
Next Document:  MicroCT with energy-resolved photon-counting detectors.