Document Detail


Magnetite/CdTe magnetic-fluorescent composite nanosystem for magnetic separation and bio-imaging.
MedLine Citation:
PMID:  21464526     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A new synthesis protocol is described to obtain a CdTe decorated magnetite bifunctional nanosystem via dodecylamine (DDA) as cross linker. High resolution transmission electron microscopy (HRTEM), energy-dispersive x-ray spectroscopy (EDAX), vibrating sample magnetometry (VSM), Fourier transform infrared spectroscopy (FTIR), diffuse reflectance spectroscopy (DRS) and fluorescence microscopy are used to characterize the constitution, size, composition and physical properties of these superparamagnetic-fluorescent nanoparticles. These CdTe decorated magnetite nanoparticles were then functionalized with anti-epidermal growth factor receptor (EGFR) antibody to specifically target cells expressing this receptor. The EGFR is a transmembrane glycoprotein and is expressed on tumor cells from different tissue origins including human leukemic cell line Molt-4 cells. The magnetite-CdTe composite nanosystem is shown to perform excellently for specific selection, magnetic separation and fluorescent detection of EGFR positive Molt-4 cells from a mixed population. Flow cytometry and confocal laser scanning microscopy results show that this composite nanosystem has great potential in antibody functionalized magnetic separation and imaging of cells using cell surface receptor antibody.
Authors:
Anup Kale; Sonia Kale; Prasad Yadav; Haribhau Gholap; Renu Pasricha; J P Jog; Benoit Lefez; Béatrice Hannoyer; Padma Shastry; Satishchandra Ogale
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Publication Detail:
Type:  Journal Article     Date:  2011-04-04
Journal Detail:
Title:  Nanotechnology     Volume:  22     ISSN:  1361-6528     ISO Abbreviation:  Nanotechnology     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-04-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101241272     Medline TA:  Nanotechnology     Country:  England    
Other Details:
Languages:  eng     Pagination:  225101     Citation Subset:  IM    
Affiliation:
National Chemical Laboratory, Council of Scientific and Industrial Research, Pune 411008, India.
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