| The macrosphere model: evaluation of a new stroke model for permanent middle cerebral artery occlusion in rats. | |
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MedLine Citation:
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PMID: 12573479 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND PURPOSE: The suture middle cerebral artery occlusion (MCAO) model is widely used for the simulation of focal cerebral ischemia in rats. This technique causes hypothalamic injury resulting in hyperthermia, which can worsen outcome and obscure neuroprotective effects. Herein, we introduce a new MCAO model that avoids these disadvantages. METHODS: Permanent MCAO was performed by intraarterial embolization using six TiO(2) macrospheres (0.3-0.4 mm in diameter) or by the suture occlusion technique. Body temperature was monitored, functional and histologic outcome was assessed after 24 h. Additional 16 rats were subjected to macrosphere or suture MCAO. Lesion progression was evaluated using magnetic resonance imaging (MRI). RESULTS: The animals subjected to suture MCAO developed hyperthermia (>39 degrees C), while the temperature remained normal in the macrosphere MCAO group. Infarct size, functional outcome and model failure rate were not significantly different between the groups. Lesion size on MRI increased within the first 90 min and remained unchanged thereafter in both groups. CONCLUSIONS: The macrosphere MCAO model provides reproducible focal cerebral ischemia, similar to the established suture technique, but avoids hypothalamic damage and hyperthermia. This model, therefore, may be more appropriate for the preclinical evaluation of neuroprotective therapies and can also be used for stroke studies under difficult conditions, e.g., in awake animals or inside the MRI scanner. |
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Authors:
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Tibo Gerriets; Fuhai Li; Matthew D Silva; Xiangjun Meng; Mathew Brevard; Christopher H Sotak; Marc Fisher |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 122 ISSN: 0165-0270 ISO Abbreviation: J. Neurosci. Methods Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-02-07 Completed Date: 2003-04-16 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 201-11 Citation Subset: IM |
Affiliation:
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Department of Neurology, University of Massachusetts Medical School, Worcester, MA 01655, USA. gerriets@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Ischemia / etiology*, physiopathology Cerebral Infarction / etiology, physiopathology Diffusion Magnetic Resonance Imaging Disease Models, Animal Hypothalamus / anatomy & histology, blood supply, injuries, physiopathology Hypothermia / etiology*, physiopathology Infarction, Middle Cerebral Artery / complications*, physiopathology* Male Microspheres* Rats Rats, Sprague-Dawley Reference Values Reproducibility of Results Sensitivity and Specificity Stroke / etiology, physiopathology Suture Techniques |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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