| mTor Plays an Important Role in Odontoblast Differentiation. | |
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MedLine Citation:
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PMID: 21763898 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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INTRODUCTION: Signaling pathways responsible for dentin regeneration in a dental pulp are not fully understood. In this study, we determined the effects of the mammalian target of rapamycin (mTor) on the differentiation and mineralization of dental pulp stem cells. We hypothesized that the two known mTor complexes Torc1 and Torc 2 play pivotal roles in the differentiation of odontoblasts and that they modulate deposition of a mineralized extracellular matrix. Therefore, we investigated the effects of Torc1 and Torc 2 signaling on the differentiation and mineralization of stem cells from human exfoliated deciduous teeth (SHED). METHODS: We used Western blot analysis to examine the expression of markers of dental differentiation in SHED (+/-) inhibition of either Torc1 or Torc 2 complex proteins raptor or rictor, respectively. In addition, the deposition of a mineralized matrix was determined under these conditions via alkaline phosphatase and alizarin red staining. RESULTS: Results show that the inhibition of Torc 1, via reduced expression of either raptor or mTor, severely restricts the synthesis of dentin sialoprotein and inhibits deposition of a mineralized matrix. Inhibition of Torc 2, via reduction of rictor, has the opposite effect, enhancing mineralization. This latter effect disappears when both rictor and mTor are inhibited, showing that the Torc 2 effect is Torc 1 dependent. CONCLUSIONS: These results strongly suggest an important role for mTor in dental pulp stem cell differentiation and provide evidence that the mechanisms involved in protein synthesis could prove an interesting target for dental pulp tissue engineering. |
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Authors:
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Jin-Koo Kim; James Baker; Jacques E Nor; Elliott E Hill |
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Publication Detail:
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Type: Journal Article Date: 2011-05-28 |
Journal Detail:
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Title: Journal of endodontics Volume: 37 ISSN: 1878-3554 ISO Abbreviation: J Endod Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7511484 Medline TA: J Endod Country: United States |
Other Details:
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Languages: eng Pagination: 1081-5 Citation Subset: D |
Copyright Information:
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Copyright © 2011 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Biology and Materials Sciences, University of Michigan, School of Dentistry, Ann Arbor, Michigan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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