Document Detail


mRNA profiling for the identification of blood--results of a collaborative EDNAP exercise.
MedLine Citation:
PMID:  20457073     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, using different kits, chemistries and instrumentation. The results demonstrate that HBB is expressed abundantly in blood, SPTB moderately and PBGD significantly less. All but one of the 16 participating laboratories were able to successfully isolate and detect RNA from the dried bloodstains even though a majority of the laboratories had no prior experience with RNA. Despite some expected variation in sensitivity between laboratories, the method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise support the potential use of mRNA profiling as an alternative to conventional serological tests.
Authors:
C Haas; E Hanson; W Bär; R Banemann; A M Bento; A Berti; E Borges; C Bouakaze; A Carracedo; M Carvalho; A Choma; M Dötsch; M Durianciková; P Hoff-Olsen; C Hohoff; P Johansen; P A Lindenbergh; B Loddenkötter; B Ludes; O Maroñas; N Morling; H Niederstätter; W Parson; G Patel; C Popielarz; E Salata; P M Schneider; T Sijen; B Sviezená; L Zatkalíková; J Ballantyne
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Publication Detail:
Type:  Journal Article     Date:  2010-02-06
Journal Detail:
Title:  Forensic science international. Genetics     Volume:  5     ISSN:  1878-0326     ISO Abbreviation:  Forensic Sci Int Genet     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101317016     Medline TA:  Forensic Sci Int Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  21-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Institute of Legal Medicine, University of Zürich, Zürich, Switzerland. cordula.haas@irm.uzh.ch
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