| mRNA levels of the differentiation-associated linker histone variant H1 zero in mitotically active and postmitotic senescent human diploid fibroblast cell populations. | |
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MedLine Citation:
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PMID: 11672986 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mRNA levels of the linker histone variant H1o, which is tightly associated with differentiation, have been studied in the present investigation in an in vitro model ageing human diploid fibroblast (HDF) cell system as a function of cumulative population doublings (CPDs) in mitotically active and senescent cell populations. According to our previous findings the synthesis rate of the H1o protein does not change as a function of CPDs as long as the cells are proliferating. However, when cells reach senescence, the synthesis rate of H1o increases in both naturally aged as well as in cell populations artificially aged by treatment with sodium butyrate. In the present investigation, it is shown that the H1o mRNA levels remain relatively constant in mitotic cells with a slight decrease in cell cultures of late CPDs, i.e. in populations which still retain a mitotic potential, but are toward the end of their proliferative lifespan. However, when cells senesce and are no longer capable of synthesizing DNA, the H1o mRNA levels increase in naturally aged cells while artificially aged cells still maintain mRNA levels comparable to those of mitotic cells. |
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Authors:
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D S Tsapali; K E Sekeri-Pataryas; T G Sourlingas |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental gerontology Volume: 36 ISSN: 0531-5565 ISO Abbreviation: Exp. Gerontol. Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-10-23 Completed Date: 2001-12-18 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: England |
Other Details:
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Languages: eng Pagination: 1649-61 Citation Subset: IM |
Affiliation:
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National Centre for Scientific Research Demokritos, Institute of Biology, Aghia Paraskevi, 153 10 Athens, Greece. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Butyrates
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pharmacology Cell Aging Cell Differentiation / physiology Cell Line Diploidy* Fibroblasts / cytology, drug effects, physiology* Histones / genetics* Humans Mitosis* RNA, Messenger / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Butyrates; 0/Histones; 0/RNA, Messenger; 0/nuclear protein H1(0) |
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