| mRNA levels of CD31, CD144, CD146 and von Willebrand factor do not serve as surrogate markers for circulating endothelial cells. | |
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MedLine Citation:
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PMID: 20589320 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Circulating endothelial cells (CEC) are considered a promising marker to determine the extent of vascular damage. However, currently available and validated CEC enumeration assays are laborious, time consuming and costly, which limits their clinical utility. Here, we evaluated the feasibility of quantifying mRNA levels of the endothelium-associated markers CD31, CD144, CD146 and von Willebrand factor (vWf) in peripheral blood (PB) of healthy donors, patients, and human umbilical veins by real-time reverse transcriptase polymerase chain reaction (RT-PCR) and their use as surrogate markers for CEC. Whole blood samples and CD146+ cell-enriched fractions were assessed for mRNA and protein expression of CD31, CD144, CD146 and vWf by RT-PCR and flow cytometry, respectively. We showed the feasibility to detect endothelial mRNA isolated from HUVEC numbers as low as 10. However, no endothelial mRNA could be measure in whole blood samples, and only low levels of CD31 and CD146 mRNA were detected in suspensions of isolated CEC with numbers up to 4,450 CEC per sample. We conclude that mRNA levels of CD31, CD144, CD146 and vWf in whole blood as detected by real time RT-PCR cannot be used as biomarkers for end-stage endothelial cells such as CEC. |
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Authors:
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Michiel H Strijbos; Brigitte A van Krimpen; Reno Debets; Jaco Kraan; Stefan Sleijfer; Jan Willem Gratama; Cor H J Lamers |
Publication Detail:
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Type: Evaluation Studies; Journal Article Date: 2010-06-29 |
Journal Detail:
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Title: Thrombosis and haemostasis Volume: 104 ISSN: 0340-6245 ISO Abbreviation: Thromb. Haemost. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-04 Completed Date: 2010-12-02 Revised Date: 2011-05-23 |
Medline Journal Info:
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Nlm Unique ID: 7608063 Medline TA: Thromb Haemost Country: Germany |
Other Details:
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Languages: eng Pagination: 318-26 Citation Subset: IM |
Affiliation:
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Laboratory of Translational Tumorimmunolgy, Department of Medical Oncology, Erasmus University Medical Center, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, CD / genetics* Antigens, CD146 / genetics Antigens, CD31 / genetics* Apoptosis Biological Markers / blood Cadherins / genetics* Cells, Cultured Endothelial Cells / metabolism*, pathology Feasibility Studies Female Flow Cytometry Humans Immunomagnetic Separation Male Middle Aged RNA, Messenger / blood* Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes / metabolism* von Willebrand Factor / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, CD146; 0/Antigens, CD31; 0/Biological Markers; 0/Cadherins; 0/MCAM protein, human; 0/RNA, Messenger; 0/cadherin 5; 0/von Willebrand Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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