Document Detail


The mERG1a channel modulates skeletal muscle MuRF1, but not MAFbx, expression.
MedLine Citation:
PMID:  23761265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: We investigated the mechanism by which the MERG1a K+ channel increases ubiquitin proteasome proteolysis (UPP).
METHODS: Hindlimb suspension and electro-transfer of Merg1a cDNA into mouse gastrocnemius muscles induced atrophy.
RESULTS: Atrophic gastrocnemius muscles of hindlimb-suspended mice express Merg1a, Murf1, and Mafbx genes. Electrotransfer of Merg1a significantly decreases muscle fiber size (12.6%) and increases UPP E3 ligase Murf1 mRNA (2.1-fold) and protein (23.7%), but does not affect Mafbx E3 ligase expression. Neither Merg1a-induced decreased fiber size nor Merg1a-induced increased Murf1 expression is curtailed significantly by coexpression of inactive HR-Foxo3a, a gene encoding a transcription factor known to induce Mafbx expression.
CONCLUSIONS: The MERG1a K+ channel significantly increases expression of Murf1, but not Mafbx. We explored this expression pattern by expressing inactive Foxo3a and showing that it is not involved in MERG1a-mediated expression of Murf1. These findings suggest that MERG1a may not modulate Murf1 expression through the AKT/FOXO pathway.
Authors:
Amber L Pond; Carrie Nedele; Wen-Horng Wang; Xun Wang; Claire Walther; Christine Jaeger; Kevin S Bradley; Huahua Du; Naoya Fujita; Gregory H Hockerman; Kevin M Hannon
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-08-30
Journal Detail:
Title:  Muscle & nerve     Volume:  49     ISSN:  1097-4598     ISO Abbreviation:  Muscle Nerve     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-02-17     Completed Date:  2014-04-17     Revised Date:  2014-06-16    
Medline Journal Info:
Nlm Unique ID:  7803146     Medline TA:  Muscle Nerve     Country:  United States    
Other Details:
Languages:  eng     Pagination:  378-88     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Ether-A-Go-Go Potassium Channels / genetics,  metabolism*
Forkhead Transcription Factors / genetics,  metabolism
Functional Laterality
Gene Expression Regulation / genetics*
Gene Transfer Techniques
Hindlimb Suspension
Male
Mice
Muscle Proteins / genetics,  metabolism*
Muscle, Skeletal
Muscular Atrophy / genetics
RNA, Messenger / metabolism
SKP Cullin F-Box Protein Ligases / genetics,  metabolism*
Time Factors
Ubiquitin-Protein Ligases / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
1R03AR053706-01A2/AR/NIAMS NIH HHS; R03 AR053706/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/ERG1 potassium channel; 0/Ether-A-Go-Go Potassium Channels; 0/Forkhead Transcription Factors; 0/FoxO3 protein, mouse; 0/Muscle Proteins; 0/RNA, Messenger; EC 6.3.2.19/Fbxo32 protein, mouse; EC 6.3.2.19/SKP Cullin F-Box Protein Ligases; EC 6.3.2.19/Trim63 protein, mouse; EC 6.3.2.19/Ubiquitin-Protein Ligases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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