Document Detail


The lysozyme from insect (Manduca sexta) is a cold-adapted enzyme.
MedLine Citation:
PMID:  17979817     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Enzymatic activity is dependent on temperature, although some proteins have evolved to retain activity at low temperatures at the expense of stability. Cold adapted enzymes are present in a variety of organisms and there is ample interest in their structure-function relationships. Lysozyme (E.C. 3.2.1.17) is one of the most studied enzymes due to its antibacterial activity against Gram positive bacteria and is also a cold adapted protein. In this work the characterization of lysozyme from the insect Manduca sexta and its activity at low temperatures is presented. Both M. sexta lysozymes natural and recombinant showed a higher content of alpha-helix secondary structure compared to that of hen egg white lysozyme and a higher specific enzymatic activity in the range of 5-30 degrees C. These results together with measured thermodynamic activation parameters support the designation of M. sexta lysozyme as a cold adapted enzyme. Therefore, the insect recombinant lysozyme is feasible as a model for structure-function studies for cold-adapted proteins.
Authors:
Rogerio R Sotelo-Mundo; Alonso A López-Zavala; Karina D Garcia-Orozco; Aldo A Arvizu-Flores; Enrique F Velázquez-Contreras; Elisa M Valenzuela-Soto; Arturo Rojo-Dominguez; Michael R Kanost
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Protein and peptide letters     Volume:  14     ISSN:  0929-8665     ISO Abbreviation:  Protein Pept. Lett.     Publication Date:  2007  
Date Detail:
Created Date:  2007-11-05     Completed Date:  2008-01-03     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  9441434     Medline TA:  Protein Pept Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  774-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Biological
Amino Acid Sequence
Animals
Cold Temperature
Manduca / enzymology*
Molecular Sequence Data
Muramidase / metabolism*
Protein Structure, Secondary
Recombinant Proteins
Sequence Alignment
Thermodynamics
Grant Support
ID/Acronym/Agency:
GM41247/GM/NIGMS NIH HHS; R01 GM041247/GM/NIGMS NIH HHS; R01 GM041247-18/GM/NIGMS NIH HHS; R01 GM041247-19/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Recombinant Proteins; EC 3.2.1.17/Muramidase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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