Document Detail

A lyophilized aqueous extract of Maytenus ilicifolia leaves inhibits histamine-mediated acid secretion in isolated frog gastric mucosa.
MedLine Citation:
PMID:  15014995     Owner:  NLM     Status:  MEDLINE    
Lyophilized aqueous extract of Maytenus ilicifolia leaves (LAEMIL) is commonly used in Brazilian folk medicine in the treatment of dyspepsia as well as gastric ulcers. We have investigated the effect and the possible mechanism of action of the LAEMIL on acid secretion in isolated frog gastric mucosa incubated in an Ussing chamber. It was observed that LAEMIL (7-28 mg%) as well as cimetidine (125-4,000 microM), a well-known histamine H2 receptor antagonist, decreased basal acid secretion in a concentration-dependent manner. Similarly to cimetidine (190 microM), LAEMIL (21 mg%) also inhibited gastric acid secretion induced by increasing concentrations of histamine (50-800 microM). The EC50 values for histamine alone and histamine in the presence of LAEMIL or cimetidine were 94.6 microM (71.1-125.9 microM), 244.9 microM (209.4-286.4 microM) and 142.2 microM (23.6-855.0 microM), respectively. LAEMIL, histamine and cimetidine were effective on acid secretion only when added to the serosal surface of the mucosa. Furthermore, simultaneous addition of LAEMIL and cimetidine at concentrations, per se, ineffective, caused a 16% reduction in the basal acid secretion [from 8.3 +/- 0.3 to 6.9 +/- 0.2 microEq g(-1) (15 min)(-1), n=4]. Although effects such as inhibition of histamine biosynthesis and/or histamine release can not be ruled out, our data suggest that LAEMIL, like cimetidine, reduces acid secretion in the isolated frog gastric mucosa by antagonising histamine H2 receptors.
Patrícia Maria Ferreira; César Nonato de Oliveira; Alaide Braga de Oliveira; Miguel José Lopes; Fernando Alzamora; Maria Aparecida Ribeiro Vieira
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-03-10
Journal Detail:
Title:  Planta     Volume:  219     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-01     Completed Date:  2004-08-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  319-24     Citation Subset:  IM    
Copyright Information:
Copyright 2004 Springer-Verlag
Department of Physiological Sciences, ICB2, Universidade Federal de Goiás, 74001-970 Goiania, GO, Brazil.
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MeSH Terms
Cimetidine / pharmacology
Dose-Response Relationship, Drug
Freeze Drying
Gastric Acid / secretion*
Gastric Mucosa / drug effects*
Histamine / pharmacology*
Hydrogen-Ion Concentration
Maytenus / chemistry*
Plant Leaves / chemistry*,  metabolism
Rana catesbeiana
Receptors, Histamine H2 / drug effects
Reg. No./Substance:
0/Receptors, Histamine H2; 51-45-6/Histamine; 51481-61-9/Cimetidine

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