Document Detail


A low fish oil inhibits SREBP-1 proteolytic cascade, while a high-fish-oil feeding decreases SREBP-1 mRNA in mice liver: relationship to anti-obesity.
MedLine Citation:
PMID:  12576519     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rodents fed fish oil showed less obesity with a reduction of triglyceride synthesis in liver, relative to other dietary oils, along with a decrease of mature form of sterol regulatory element binding protein-1 (SREBP-1) and activation of peroxisome proliferator-activated receptor alpha (PPARalpha). Decrease of mature SREBP-1 protein by fish oil feeding was due to either inhibition of SREBP-1 proteolytic cascade or to decrease of its mRNA. To clarify its mechanism and relation to antiobesity effect, mice were fed fish oil in a range from 10 to 60 energy percent (en%). Fish oil feeding decreased body weight and fat mass in a dose-dependent manner, in parallel with PPARalpha activation and a decrease of SREBP-1 mRNA. However, compared with 0 en% fish oil feeding, 10 en% fish oil feeding decreased mature SREBP-1 protein by 50% with concomitant decreases of lipogenic genes, while precursor SREBP-1 protein rather increased by 1.3-fold. These data suggest that physiological doses of fish oil feeding effectively decrease expression of liver lipogenic enzymes by inhibiting SREBP-1 proteolytic cascade, while substantial decrease of SREBP-1 expression is observed in its pharmacological doses, and that activation of PPARalpha rather than SREBP-1 decrease might be related to the antiobesity effect of fish oil feeding.
Authors:
Teruyo Nakatani; Hyoun-Ju Kim; Yasushi Kaburagi; Kazuki Yasuda; Osamu Ezaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-11-16
Journal Detail:
Title:  Journal of lipid research     Volume:  44     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-10     Completed Date:  2004-02-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  369-79     Citation Subset:  IM    
Affiliation:
Division of Clinical Nutrition, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acetyl-CoA Carboxylase / genetics
Adipose Tissue / physiology
Animals
Body Weight
CCAAT-Enhancer-Binding Proteins / genetics,  metabolism*
DNA-Binding Proteins / genetics,  metabolism*
Dietary Fats / administration & dosage,  metabolism
Fatty Acid Synthetase Complex / genetics
Female
Fish Oils / administration & dosage*,  metabolism
Liver / chemistry,  cytology,  metabolism,  physiology*
Mice
Mice, Inbred C57BL
Muscle, Skeletal / physiology
Obesity / metabolism*
Organ Size
RNA, Messenger / metabolism*
Receptors, Cytoplasmic and Nuclear / metabolism
Stearoyl-CoA Desaturase / genetics
Sterol Regulatory Element Binding Protein 1
Subcellular Fractions / chemistry,  metabolism
Transcription Factors / metabolism
Chemical
Reg. No./Substance:
0/CCAAT-Enhancer-Binding Proteins; 0/DNA-Binding Proteins; 0/Dietary Fats; 0/Fish Oils; 0/RNA, Messenger; 0/Receptors, Cytoplasmic and Nuclear; 0/Srebf1 protein, mouse; 0/Sterol Regulatory Element Binding Protein 1; 0/Transcription Factors; EC 1.14.19.1/Stearoyl-CoA Desaturase; EC 6.-/Fatty Acid Synthetase Complex; EC 6.4.1.2/Acetyl-CoA Carboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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