Document Detail

A low-cost, scalable technique to study distal coronary arteriole function.
MedLine Citation:
PMID:  24698112     Owner:  NLM     Status:  Publisher    
AIM: Although most coronary heart disease is due to atherosclerosis, 10-20 % of patients with cardiac chest pain have normal epicardial arteries, indicating dysfunction of the resistance vessels. Present techniques to study arteriole function involve microsurgery and microperfusion; expensive techniques in terms of equipment and training. To provide a low-cost alternative we adapted the agarose preparation, originally developed for renal vessels, to isolate heart resistance vessels.
METHODS: The coronary circulation of mice and rats was infused with warmed agarose, which was solidified to create a tensile intravascular core substituting the need for later pressurization. Slices of the right ventricle were enzyme-treated to liberate vessels. Fragments identified as distal coronary arterioles by the presence of branching capillaries were mounted by self-adhesion in a microscope perfusion chamber.
RESULTS: Vessel morphology was excellent and contractility was consistent with low standard errors of mean. Phenylephrine contracted arterioles to relative diameter 0.82±0.05 (n=4, baseline lumen diameter (BLD) 25±1 μm) and 0.68±0.04 (n=5, BLD 20±1 μm) from rats and mice, respectively (p<0.05, 10(-4) M). Subsequent depolarization with 50 mM KCl contracted vessels to 0.82±0.03 and 0.71±0.03, respectively (p<0.05). ANG II contracted mouse arterioles to 0.79±0.06 (n=4, BLD 24±2 μm, 10(-7) M). KCl produced a fura-2 ratio increase of 0.21±0.03 in mouse vessels (n=8, 50 mM).
CONCLUSION: The agarose preparation is an inexpensive technique that provides access to the distalmost region of the coronary arteriole. The preparation is ideal to compare function and signalling in coronary arterioles harvested from hearts with different physiology, pathology or drug-treatment. This article is protected by copyright. All rights reserved.
Frank Helle; Tone Dolva Dahl; Christos Chatziantoniou
Publication Detail:
Type:  EDITORIAL     Date:  2014-4-3
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  -     ISSN:  1748-1716     ISO Abbreviation:  Acta Physiol (Oxf)     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2014-4-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
This article is protected by copyright. All rights reserved.
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