Document Detail

A low-cost, rapid deposition method for "smart" films: applications in mammalian cell release.
MedLine Citation:
PMID:  20423125     Owner:  NLM     Status:  MEDLINE    
The "smart" polymer poly (N-isopropyl acrylamide), or pNIPAM, has been studied for bioengineering applications. The polymer's abrupt change in hydrophobicity near physiologic temperatures makes it ideal for use as a substrate in many applications, including protein separation and prevention of biofouling. To tether pNIPAM, many techniques such as plasma deposition, have been utilized, but most are expensive and require long equipment calibration or fabrication periods. Recently, a novel method for codepositing this smart polymer with a sol-gel, tetraethyl orthosilicate (TEOS), was developed. In this work, we adapt this technique for applications in mammalian cell attachment/detachment. In addition, we compare the effects of the pNIPAM/TEOS ratio to functionality using surface analysis techniques (XPS and contact angles). We found the optimal ratio to be 0.35 wt % pNIPAM/TEOS. Cell detachment from these substrates indicate that they would be ideal for applications that do not require intact cell sheets, such as biofouling prevention and protein separation, as this technique is a simple and affordable technique for pNIPAM deposition.
Jamie A Reed; Adrianne E Lucero; Steve Hu; Linnea K Ista; Mangesh T Bore; Gabriel P L?pez; Heather E Canavan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  ACS applied materials & interfaces     Volume:  2     ISSN:  1944-8244     ISO Abbreviation:  ACS Appl Mater Interfaces     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-28     Completed Date:  2010-06-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101504991     Medline TA:  ACS Appl Mater Interfaces     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1048-51     Citation Subset:  IM    
Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, New Mexico 87131-1141, USA.
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MeSH Terms
Acrylic Resins / chemistry*
Cell Adhesion
Cell Culture Techniques* / methods
Cell Line
Hot Temperature
Materials Testing
Phase Transition
Photoelectron Spectroscopy / methods*
Silanes / chemistry
Surface Properties
Tissue Engineering / methods
Reg. No./Substance:
0/Acrylic Resins; 0/Silanes; 25189-55-3/poly-N-isopropylacrylamide; 78-10-4/tetraethoxysilane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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