Document Detail

The loss of gap junctional cell-to-cell communication is coupled with dedifferentiation of retinal pigmented epithelial cells in the course of transdifferentiation into the lens.
MedLine Citation:
PMID:  7981045     Owner:  NLM     Status:  MEDLINE    
Retinal pigmented epithelial cells (PECs) of the chick embryo can be cultured as a monolayer of melanized hexagonal cells. Modifications of the culture condition make the cells lose most of the phenotypes and further transdifferentiate into lentoid bodies within a few weeks. Ultrastructural observations showed that PECs and the lentoids have gap junctions with distinct morphology. Diffusion of a fluorescent dye confirmed the presence of gap junctions in both phenotypes. However, cells in the intermediate stage of transdifferentiation, which show neither the phenotype of the PEC nor that of the lentoid and are called dedifferentiated PECs here, have almost no gap junction structure. We propose the possibility that the dedifferentiation of PECs and the loss of cell-to-cell communication are tightly coupled events. This cell culture system is a suitable material for further studying this relationship by cellular and molecular approaches.
R Kodama; G Eguchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of developmental biology     Volume:  38     ISSN:  0214-6282     ISO Abbreviation:  Int. J. Dev. Biol.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1995-01-05     Completed Date:  1995-01-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8917470     Medline TA:  Int J Dev Biol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  357-64     Citation Subset:  IM    
Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan.
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MeSH Terms
Cell Communication
Cell Differentiation
Cells, Cultured
Chick Embryo
Fluorescent Dyes
Gap Junctions / ultrastructure
Lens, Crystalline / cytology,  embryology
Microscopy, Electron
Pigment Epithelium of Eye / cytology*,  embryology
Reg. No./Substance:
0/Fluorescent Dyes; 0/Isoquinolines; 77944-88-8/lucifer yellow

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