Document Detail


A longitudinal study of gastrointestinal parasites in Canadian dairy farms. The value of an indirect Ostertagia ostertagi ELISA as a monitoring tool.
MedLine Citation:
PMID:  12127251     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The general objective of this study was to evaluate a crude Ostertagia ostertagi antigen enzyme-linked immunosorbent assay (ELISA) for monitoring gastrointestinal parasites in lactating dairy cattle. A longitudinal study of gastrointestinal parasites in lactating dairy cows was carried out in 38 herds in four provinces of Canada (Prince Edward Island, Quebec, Ontario and Saskatchewan) from September 1999 to October 2000. Bulk tank milk, cow milk, serum and fecal samples were collected monthly or quarterly from all these farms. Information on herd management factors was collected by a standard questionnaire and individual cow production data were obtained from an electronic database. The overall mean optical density ratio (ODR) was 0.30 and ranged from -0.05 to 1.55. Although a clear seasonal pattern was not observed, the ODR values tended to decrease during the housing period and start increasing in the spring before the cows went out to pasture. The second and third or greater lactation cows had significantly higher ODR values compared with first lactation animals. The individual cow ODR had a very low correlation with individual squared root fecal egg counts but showed a reasonably high correlation when herd averages values were computed (r=0.73). A moderate correlation (r approximately 0.50) between the bulk tank and herd average ODR was observed. Milk yield was negatively associated with individual cow milk ODR and a quadratic effect on ODR was observed for days in milk. Twenty-eight of the herds participated in a clinical trial of eprinomectin (Ivomec Eprinex) treatment at calving. The cow level ODR values determined late in the previous lactation had a marginally significant effect (P=0.07) on treatment response, suggesting that high OD cows responded better to the anthelmintic treatment. However, because of the small sample size available in this model, more research is needed to better understand this relationship. In conclusion, the indirect ELISA using an O. ostertagi crude antigen appears useful as a technique for monitoring gastrointestinal parasite burdens in adult dairy cows and holds promise as a potential predictor of response to anthelmintic treatment.
Authors:
Javier Sanchez; Ian Dohoo; Ane Nødtvedt; Greg Keefe; Fred Markham; Ken Leslie; Luc DesCôteaux; John Campbell
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Veterinary parasitology     Volume:  107     ISSN:  0304-4017     ISO Abbreviation:  Vet. Parasitol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-19     Completed Date:  2002-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7602745     Medline TA:  Vet Parasitol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  209-26     Citation Subset:  IM    
Affiliation:
Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, PEI, C1A 4P3, Charlottetown, Canada. jsanchez@upei.ca
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MeSH Terms
Descriptor/Qualifier:
Animal Husbandry
Animals
Anthelmintics / therapeutic use
Antibodies, Helminth / blood
Antigens, Helminth / immunology
Canada
Cattle
Cattle Diseases / diagnosis,  drug therapy,  parasitology*
Enzyme-Linked Immunosorbent Assay / veterinary*
Feces / parasitology
Female
Gastrointestinal Diseases / diagnosis,  drug therapy,  parasitology,  veterinary*
Ivermectin / analogs & derivatives*,  therapeutic use
Longitudinal Studies
Milk / parasitology,  secretion
Multivariate Analysis
Ostertagia* / immunology,  parasitology
Parasite Egg Count
Chemical
Reg. No./Substance:
0/Anthelmintics; 0/Antibodies, Helminth; 0/Antigens, Helminth; 160902-72-7/4''-epiacetylamino-4''-deoxyavermectin B1; 70288-86-7/Ivermectin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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