| The long-term culture of encapsulated pancreatic islets. | |
| | |
MedLine Citation:
|
PMID: 2430818 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A new encapsulation technique, the formation of a polyelectrolyte complex membrane from sodium cellulose sulphate and a polycationic solution (polydimethyldiallylammoniumchloride) can be used for culturing pancreatic islets. After 5 weeks of culture, such encapsulated islets were morphologically well preserved, and parameters of cell viability and functionality such as DNA content and DNA synthesis, insulin content and stimulated insulin release were not different between the encapsulated and non-encapsulated control islets. A lower insulin secretion into the medium of the encapsulated islets during the first 3 weeks of culture can probably be explained by the capsule diameter (4-5 mm) and should be improved using microcapsules with a diameter of less than 1 mm. |
| | |
Authors:
|
K Braun; W Besch; S Lucke; H J Hahn |
Related Documents
:
|
1994378 - Expression of fetoacinar pancreatic (fap) protein in the pancreatic human tumor cell li... 11570968 - Changes in the relative abundance of mrna transcripts for insulin-like growth factor (i... 1730818 - Comparative studies of the erythroid-potentiating effects of biosynthetic human insulin... 10857768 - Lymphokine release from human lymphomononuclear cells after co-culture with isolated pa... 21803898 - Induction of attachment-independent biofilm formation and repression of hfq expression ... 19428818 - Comparison of alterations in amino acids content in cultured astrocytes or neurons expo... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Experimental and clinical endocrinology Volume: 87 ISSN: 0232-7384 ISO Abbreviation: Exp. Clin. Endocrinol. Publication Date: 1986 Aug |
Date Detail:
|
Created Date: 1987-01-02 Completed Date: 1987-01-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8302802 Medline TA: Exp Clin Endocrinol Country: GERMANY, EAST |
Other Details:
|
Languages: eng Pagination: 313-8 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
1-Methyl-3-isobutylxanthine
/
pharmacology Animals Capsules Cellulose / analogs & derivatives Culture Techniques / methods* DNA / metabolism Insulin / secretion Islets of Langerhans / anatomy & histology*, drug effects, physiology Polyamines* Polymers Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
|
0/Capsules; 0/Polyamines; 0/Polymers; 0/polycations; 11061-68-0/Insulin; 28822-58-4/1-Methyl-3-isobutylxanthine; 9004-34-6/Cellulose; 9007-49-2/DNA; 9032-43-3/cellulose sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Influence of substance P on ciliary beat frequency in airway isolated preparations.
Next Document: The in vitro lifespan of MRC-5 cells is shortened by 5-azacytidine-induced demethylation.