Document Detail


lmmunoelectron microscopic examination of cord blood CD5+ B cells.
MedLine Citation:
PMID:  17142958     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we have categorized cord blood CD5(+) B cells, which were examined using the horseradish peroxidase-colloidal gold double labeling immunoelectron microscopy, into three subtypes based on their morphology and immunohistochemical characteristics. Type 1a cells and type 1b cells (9% and 17% of the CD5(+) B cells, respectively) had few cytoplasmic organelles, a high nuclear/cytoplasmic (N/C) ratio (0.66 +/- 0.03 and 0.58 +/- 0.04, respectively), and a low nuclear contour index (NCI) value (1.56 +/- 0.30 and 1.50 +/- 0.27, respectively), whereas type 2 cells (74% of the CD5(+) B cells) had a low N/C ratio (0.44 +/- 0.11) and a high NCI value (2.05 +/- 0.68). Type 2 cells, which had many cytoplasmic organelles, frequently had several uropod-like processes that bound to the gold particles. The N/C ratios clearly showed that there were significant differences among the three types of CD5(+) B cells (p < 0.01), and between CD5(+) T cells and the three types of CD5(+) B cells (p < 0.05). For the NCI values, only type 1b and type 2 cells showed a significant difference (p < 0.05). These findings suggest that type 1a cells are transformed into type 1b cells, and then into type 2 cells.
Authors:
Shigeyuki Asano; Haruki Wakasa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical and experimental hematopathology : JCEH     Volume:  46     ISSN:  1346-4280     ISO Abbreviation:  J Clin Exp Hematop     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-12-04     Completed Date:  2007-01-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101141257     Medline TA:  J Clin Exp Hematop     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  83-8     Citation Subset:  IM    
Affiliation:
Division of Pathology, Iwaki Kyoritsu, General Hospital, Iwaki, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD5 / metabolism*
B-Lymphocyte Subsets / immunology,  metabolism,  ultrastructure*
B-Lymphocytes / immunology,  metabolism,  ultrastructure*
Cell Differentiation / immunology
Fetal Blood / cytology*,  immunology
Humans
Immunohistochemistry
Microscopy, Immunoelectron
Chemical
Reg. No./Substance:
0/Antigens, CD5

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