Document Detail


The liver mediates apoptotic cell-induced immune regulation.
MedLine Citation:
PMID:  18782258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Allogeneic apoptotic cells have been demonstrated to induce allograft tolerance, but the mechanisms for this remain unclear. The study presented here investigates organs in which the tolerogenic immune responses may occur. Distribution of live or apoptotic CFSE(+) splenocytes in recipients' organs and phagocytosis by liver antigen-presenting cells (APC) were investigated by fluorescence microscopy and flow cytometry, and cytokine expression was analysed by Multiplex and ELISA. It was found that allogeneic or autogenic apoptotic cells preferentially accumulated in the liver within 30 min, peaked at 60 min, and disappeared at 12 h after infusion, whereas these cells scarcely appeared in the spleen. The accumulation in the liver was apoptotic cell-specific as both allogeneic and autogenic live splenocytes were completely deposited in the spleen. Liver phagocytes, including Kupffer cells, liver sinusoidal endothelial cells and dendritic cells, all efficiently phagocytized apoptotic cells in vitro and in vivo. Although a Th1 cytokine profile found both in the spleen and liver in the recipients of allogeneic apoptotic cells, a rapid and consistent Th2 cytokine profile specifically was initiated in the liver. From this, we conclude that liver APC phagocytize donor apoptotic cells and induce liver-specific Th2 cytokines, which may contribute to the mechanisms of allograft tolerance induced by donor apoptotic cells.
Authors:
Y Wang; Y Gao; X Yuan; W Xia; Y Luo; E Sun; Z K Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of immunology     Volume:  68     ISSN:  1365-3083     ISO Abbreviation:  Scand. J. Immunol.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-10     Completed Date:  2008-10-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0323767     Medline TA:  Scand J Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  297-305     Citation Subset:  IM    
Affiliation:
Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen-Presenting Cells / immunology*
Apoptosis / immunology*
Cell Movement
Cytokines / biosynthesis
Immune Tolerance
Liver / cytology,  immunology*,  metabolism
Lymphocyte Transfusion
Lymphocytes / physiology
Male
Mice
Mice, Inbred C57BL
Phagocytosis
Spleen / cytology,  immunology
Time Factors
Transforming Growth Factor beta1 / biosynthesis
Transplantation, Homologous
Chemical
Reg. No./Substance:
0/Cytokines; 0/Transforming Growth Factor beta1

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