Document Detail


A liquid core waveguide fluorescence detector for multicapillary electrophoresis applied to DNA sequencing in a 91-capillary array.
MedLine Citation:
PMID:  11001228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new laser-induced fluorescence (LIF) detector for multicapillary electrophoresis is presented. The detection principle is based on waveguiding of the emitted fluorescence from the point of illumination to the capillary ends by total internal reflection (TIR) and imaging of the capillary ends. The capillaries themselves thus act as liquid core waveguides (LCWs). At the illumination point, the capillaries are arranged in a planar array, which allows clean and efficient illumination with a line-focused laser beam. The capillary ends are rearranged into a small, densely packed two-dimensional array, which is imaged end-on with high light collection efficiency and excellent image quality. Wavelength dispersion is obtained with a single prism. Intercapillary optical crosstalk is less than 0.5%, and rejection of stray light is very efficient. The detector is applied to four-color DNA sequencing by gel electrophoresis in a 91-capillary array, with simple fluorescein and rhodamine dyes as fluorophores. Since the imaged two-dimensional array is so compact, the detector has a high potential for very large-scale multiplexing.
Authors:
A Hanning; J Westberg; J Roeraade
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  21     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2001-01-18     Completed Date:  2001-01-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  3290-304     Citation Subset:  IM    
Affiliation:
Royal Institute of Technology, Department of Analytical Chemistry, Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Capillarity
DNA / chemistry*
Electrophoresis, Capillary / instrumentation*
Equipment Design
Image Processing, Computer-Assisted
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis / instrumentation,  methods*
Spectrometry, Fluorescence / instrumentation,  methods
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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