Document Detail


A lipobead microarray assembled by particle entrapment in a microfluidic obstacle course and used for the display of cell membrane receptors.
MedLine Citation:
PMID:  23748734     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Platforms which can display cell membrane ligands and receptors as a microarray library of probes for screening against a target are essential tools in drug discovery, biomarker identification, and pathogen detection. Membrane receptors and ligands require their native bilayer environment to retain their selectivity and binding affinity, and this complicates displaying them in a microarray platform. In this study, a design is developed in which the probes are first incorporated in supported lipid bilayers formed around micron-sized particles (lipobeads), and the microbeads themselves are then arrayed on a surface by hydrodynamic capture in a microfluidic obstacle course of traps. The traps are "V" shaped open enclosures, which are arranged in a wide channel of a microfluidic device, and capture the lipobeads (slightly smaller than the channel height) as they are streamed through the course. Screening assays are undertaken directly in the device after assembly, by streaming a fluorescently labeled target through the device and detecting the bead fluorescence. Conditions are first established for which the supported bilayers on the bead surface remain intact during the capture and assay steps, using fluorescent tags in the bilayer to infer bilayer integrity. Numerical calculations of the hydrodynamic drag coefficient on the entrapped beads are presented in conjunction with the stability experiments to develop criteria for the bilayer stability as a function of the screening assay perfusion rate. Simulations of the flow streamlines are also presented to quantify the trapping efficiency of the obstacle course. Screening assays are illustrated, assaying fluorescently labeled NeutrAvidin with biotin, and labeled cholera toxin with its ganglioside binding ligand, GM1. Sequential capturing of sets of lipobeads (one at a time, and with each set bearing a different probe), followed by indexing the bead positions after each set is entrapped, allows for the construction of an indexed array of multiple probes without the need for particle encoding and is illustrated using the NeutrAvidin-biotin pair. Finally, the lipobead platform is used for quantitatively measuring the kinetic rate constants for the binding of a probe (biotin) to a target (NeutrAvidin).
Authors:
Xiaoxiao Chen; Shahab Shojaei-Zadeh; M Lane Gilchrist; Charles Maldarelli
Related Documents :
22783804 - Blastomyces dermatitidis septins cdc3, cdc10, and cdc12 impact the morphology of yeast ...
24340194 - Projected marine climate change: effects on copepod oxidative status and reproduction.
22362144 - Phase behavior of phytosterol ethoxylates in an imidazolium-type room-temperature ionic...
23963614 - Nano-pipette directed transport of nanotube transmembrane channels and hybrid vesicles.
9237304 - Temperature effects on the breeding distribution of grey seals (halichoerus grypus).
10926664 - Central histamine contributed to temperature-induced polypnea in mice.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-6-7
Journal Detail:
Title:  Lab on a chip     Volume:  -     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-6-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Levich Institute and Department of Chemical Engineering, The City College of the City University of New York, New York, New York 10031, U. S. A. charles@chemail.engr.ccny.cuny.edu.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Transrenal Ureteral Occlusion Using the Amplatzer Vascular Plug II: A New Interventional Treatment O...
Next Document:  Forefoot strikers exhibit lower running-induced knee loading than rearfoot strikers.