| A lipid-mediated quality control process in the Golgi apparatus in yeast. | |
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MedLine Citation:
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PMID: 18094052 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When heme biosynthesis is disrupted, the yeast Saccharomyces cerevisiae becomes unable to synthesize its major sterol, ergosterol, and desaturate fatty acids. We took advantage of this physiological peculiarity to evaluate the consequences of ergosterol and/or unsaturated fatty acid (UFA) depletions on the biogenesis of a model polytopic plasma membrane protein, the uracil permease Fur4p. We show that under UFA shortage, which results in low amounts of diunsaturated phospholipid species, and under ergosterol depletion, Fur4p is prematurely routed from the Golgi apparatus to the vacuolar lumen in a process that requires the ubiquitin ligase Rsp5p. Interestingly, this diversion is not correlated to Fur4p exclusion from detergent-resistant membranes. In an independent set of experiments, we show that Fur4p targeting to the plasma membrane depends on phosphatidylethanolamine amounts and more specifically on the propensity of this phospholipid to form a hexagonal phase. In light of recent literature, we propose a model in which ergosterol and diunsaturated phospholipid species maintain optimal membrane curvature for Fur4p to evade the Golgi quality control process and to be properly delivered to its normal destination. |
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Authors:
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Ludovic Pineau; Laetitia Bonifait; Jean-Marc Berjeaud; Parissa Alimardani-Theuil; Thierry Bergès; Thierry Ferreira |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-19 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 19 ISSN: 1939-4586 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-03 Completed Date: 2008-11-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 807-21 Citation Subset: IM |
Affiliation:
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Université de Poitiers, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6161 Transport des assimilats 86022 Poitiers Cedex, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Membrane
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metabolism Endosomes / metabolism Ergosterol / metabolism Fatty Acids / metabolism Fatty Acids, Unsaturated / metabolism Golgi Apparatus / metabolism* Green Fluorescent Proteins / metabolism Lipid Metabolism* Nucleotide Transport Proteins / metabolism Phosphatidylcholines / metabolism Phosphatidylethanolamines / metabolism Protein Transport Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae / cytology, growth & development, metabolism* Saccharomyces cerevisiae Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/FUR4 protein, S cerevisiae; 0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Nucleotide Transport Proteins; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Recombinant Fusion Proteins; 0/Saccharomyces cerevisiae Proteins; 147336-22-9/Green Fluorescent Proteins; 57-87-4/Ergosterol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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