| The lipid A from Vibrio fischeri lipopolysaccharide: a unique structure bearing a phosphoglycerol moiety. | |
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MedLine Citation:
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PMID: 21498521 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vibrio fischeri, a bioluminescent marine bacterium, exists in an exclusive symbiotic relationship with the Hawaiian bobtail squid, Euprymna scolopes, whose light organ it colonizes. Previously, it has been shown that the lipopolysaccharide (LPS) or free lipid A of V. fischeri can trigger morphological changes in the juvenile squid's light organ that occur upon colonization. To investigate the structural features that might be responsible for this phenomenon, the lipid A from V. fischeri ES114 LPS was isolated and characterized by multistage mass spectrometry (MS(n)). A microheterogeneous mixture of mono- and diphosphorylated diglucosamine disaccharides was observed with variable states of acylation ranging from tetra- to octaacylated forms. All lipid A species, however, contained a set of conserved primary acyl chains consisting of an N-linked C14:0(3-OH) at the 2-position, an unusual N-linked C14:1(3-OH) at the 2'-position, and two O-linked C12:0(3-OH) fatty acids at the 3- and 3'-positions. The fatty acids found in secondary acylation were considerably more variable, with either a C12:0 or C16:1 at the 2-position, C14:0 or C14:0(3-OH) at the 2'-position, and C12:0 or no substituent at the 3'-position. Most surprising was the presence of an unusual set of modifications at the secondary acylation site of the 3-position consisting of phosphoglycerol (GroP), lysophosphatidic acid (GroP bearing C12:0, C16:0, or C16:1), or phosphatidic acid (GroP bearing either C16:0 + C12:0 or C16:0 + C16:1). Given their unusual nature, it is possible that these features of the V. fischeri lipid A may underlie the ability of E. scolopes to recognize its symbiotic partner. |
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Authors:
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Nancy J Phillips; Dawn M Adin; Eric V Stabb; Margaret J McFall-Ngai; Michael A Apicella; Bradford W Gibson |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-04-15 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 286 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-06-13 Completed Date: 2011-08-30 Revised Date: 2012-01-09 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 21203-19 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aliivibrio fischeri
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metabolism* Animals Decapodiformes / microbiology* Fatty Acids / chemistry Gas Chromatography-Mass Spectrometry / methods Glycerophosphates / chemistry Lipid A / chemistry* Lipopolysaccharides / metabolism* Models, Chemical Phosphatidic Acids / chemistry Phosphorylation Spectrometry, Mass, Electrospray Ionization / methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Symbiosis Tandem Mass Spectrometry / methods |
| Grant Support | |
ID/Acronym/Agency:
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AI-50661/AI/NIAID NIH HHS; P30 CA082103/CA/NCI NIH HHS; R01 AI024616-23/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Glycerophosphates; 0/Lipid A; 0/Lipopolysaccharides; 0/Phosphatidic Acids; 57-03-4/alpha-glycerophosphoric acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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