Document Detail


The lipid composition of isolated brain cells and axons.
MedLine Citation:
PMID:  1223319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The current status of the published work on the lipid composition of isolated brain cells is reviewed and some new work on the sphingolipids of these cells is presented. In spite of considerable differences in isolation techniques between different groups, the lipid analyses of different cell preparations are similar enough to permit several generalizations. This fact is an encouraging sign that cell separation methods have considerable usefulness in defining the composition of normal brain cells. It is a general finding that astrocytes have more lipid than neuronal perikarya but that the gross lipid composition of these two cell types is surprisingly similar. Oligodendroglial lipids are quite different from those of the other two cell types and are characterized by a high galactolipid content. Although such a lipid pattern might be expected in oligodendroglia, which are myelin-forming cells, axonal lipids have an even higher galactolipid content. In an effort to find more cell-specific patterns, the glycosphingolipids were examined in more detail. Differences were seen in the distribution and fatty-acid patterns of these minor lipids in neurons and astrocytes, although it may be premature to conclude that these differences will prove to be cell-specific. All of the isolated cells were found to contain galactosylceramide, sulfatide, glucosylceramide, dihexosylceramide, and gangliosides. The distribution of these lipids in the normal cells was found to differ considerably from that reported in cultured neuroblastoma cells or astrocytoma cells. Not only were gangliosides present in all cells but the ganglioside patterns of neurons and astrocytes were nearly identical. The fatty-acid patterns of the neuronal and astroglial sphingolipids generally do not resemble each other, and both are quite different from those found in oligodendroglia and axons. However, the fatty-acid composition of the sphingolipids from bovine oligodendroglia and from axons are similar and resemble those of myelin lipids. The fatty acids of glucosylceramide and dihexosylceramide are similar in all three cell types. They have rather large amounts of 16:0 and acids longer than C18; thus they are considerably different from the ganglioside fatty acids (which have mostly 15:0) isolated from the same fractions.
Authors:
W T Norton; T Abe; S E Poduslo; G H DeVries
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  1     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  1975  
Date Detail:
Created Date:  1976-07-06     Completed Date:  1976-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  57-75     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Astrocytes / analysis
Astrocytoma / analysis
Axons / analysis
Brain / cytology*
Brain Chemistry*
Brain Neoplasms / analysis
Cattle
Fatty Acids / analysis
Gangliosides / analysis
Glycosphingolipids / analysis
Lipids / analysis*
Mice
Neurons / analysis
Oligodendroglia / analysis
Rabbits
Rats
Swine
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Gangliosides; 0/Glycosphingolipids; 0/Lipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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