Document Detail

The linkage region in the polypeptide of pig costal cartilage proteoglycan.
MedLine Citation:
PMID:  126839     Owner:  NLM     Status:  MEDLINE    
Proteoglycan from pig costal cartilage and fragments obtained by proteolytic digestion were characterized by equilibrium ultracentrifugation and amino acid analysis. The proteoglycan extractable in 4 M guanidinium chloride yielded, after proteolytic digestion with trypsin and chymotrypsin, a chondroitin sulfate peptide containing four chains of polysaccharide. The unextractable residue yielded chondroitin sulfate peptide containing only two chains. The amino acid composition indicated a fairly uniform spacing between all four chains with an average of eight amino acid residues between the serine residues involved in linkage. Following the alkaline sulfite elimination-addition reaction, free peptide was isolated and found to contain one unsubstituted serine residue for every two linked glycosidically. Glycine and glutamic acid were the only two amino acids sufficiently abundant to be part of an invariant sequence near to serine residues destined to be glycosylated. The linkage region of the polypeptide also contains some substituted serine residues which do not carry a full chondroitin sulfate chain.
F S Wusteman; E A Davidson
Related Documents :
17395169 - Structural relation of the antigenic polysaccharides of escherichia coli o40, shigella ...
23543349 - T cell responses to citrullinated self-peptides in patients with rheumatoid arthritis.
12492399 - Biosynthesis of heparan sulphate with diverse structures and functions: two alternative...
2590169 - Dermatan sulphate proteoglycans of human articular cartilage. the properties of dermata...
9492329 - Structural determination of the o-antigenic polysaccharide from the enterotoxigenic esc...
16866359 - Chemical mechanism and specificity of the c5-mannuronan epimerase reaction.
20671109 - Identification and characterization of in vitro galactosyltransferase activities involv...
12220669 - Identification of a basic surface area of the fadd death effector domain critical for a...
1920439 - Structural and thermodynamic analysis of the packing of two alpha-helices in bacterioph...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Connective tissue research     Volume:  3     ISSN:  0300-8207     ISO Abbreviation:  Connect. Tissue Res.     Publication Date:  1975  
Date Detail:
Created Date:  1976-01-26     Completed Date:  1976-01-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0365263     Medline TA:  Connect Tissue Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  123-33     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Amino Acids / analysis
Cartilage* / analysis
Chondroitin / analogs & derivatives*
Chondroitin Sulfates* / analysis
Molecular Weight
Peptides / analysis
Protein Conformation
Proteoglycans* / analysis
Serine / analysis
Xylose / analysis
Reg. No./Substance:
0/Amino Acids; 0/Glycosaminoglycans; 0/Peptides; 0/Proteoglycans; 0/Xylose; 56-45-1/Serine; 9007-27-6/Chondroitin; 9007-28-7/Chondroitin Sulfates; EC; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Stimulation of chondroitin sulfate proteoglycan production by chondrocytes in monolayer.
Next Document:  Studies on the mechanism of hyaluronate lyase action.