| The linkage of lysine in the O-specific chains of Proteus mirabilis 1959. | |
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MedLine Citation:
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PMID: 767106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipopolysaccharides of qualitatively identical but quantitatively different sugar composition were extracted from Proteus mirabilis strain 1959. The lipopolysaccharide with the higher percentage of typical O-specific constituents was subjected to partial acid hydrolysis. An oligosaccharide B22 was separated by paper chromatography and electrophoresis. It was found to be composed of equimolar amounts of D-galacturonic acid, D-galactosamine and L-lysine. Dinitrophenylation of the oligosaccharide as well as of the genuine lipopolysaccharide afforded xi-dinitrophenyl-L-lysine after acid hydrolysis, showing that lysine was linked to the disaccharide via its alpha-amino group. Further studies including the Morgan-Elson and Elson-Morgan reactions, NaBH4-reduction, hydrazinolysis and periodate oxidation revealed the structure of oligosaccharide B22 as D-galacturonyl-(1 leads to 4)-D-galactosamine with lysine attached to the carboxylic group of galacturonic acid via its alpha-amino group. Judged from its high inhibition capacity this oligosaccharide has to be considered as an essential part of the serological determinant of Proteus mirabilis 1959. The frequent occurrence of lysine and galacturonic acid in Proteus mirabilis O-serogroups and their possible significance for the respective serological specificities are discussed. |
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Authors:
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W Gromska; H Mayer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 62 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1976 Feb |
Date Detail:
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Created Date: 1976-06-02 Completed Date: 1976-06-02 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 391-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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ABO Blood-Group System* Animals Antigens, Bacterial* Binding Sites Complement Fixation Tests Glucose / analysis Hexosamines / analysis Hydrazines Immunoelectrophoresis Lysine / metabolism Mannose / analysis Oligosaccharides / pharmacology Polysaccharides, Bacterial* / immunology Proteus mirabilis / analysis* Rabbits / immunology Uronic Acids / analysis |
| Chemical | |
Reg. No./Substance:
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0/ABO Blood-Group System; 0/Antigens, Bacterial; 0/Hexosamines; 0/Hydrazines; 0/Oligosaccharides; 0/Polysaccharides, Bacterial; 0/Uronic Acids; 31103-86-3/Mannose; 50-99-7/Glucose; 56-87-1/Lysine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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