| lines and bowl affect the specification of cyst stem cells and niche cells in the Drosophila testis. | |
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MedLine Citation:
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PMID: 21486923 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To function properly, tissue-specific stem cells must reside in a niche. The Drosophila testis niche is one of few niches studied in vivo. Here, a single niche, comprising ten hub cells, maintains both germline stem cells (GSC) and somatic stem cells (CySC). Here, we show that lines is an essential CySC factor. Surprisingly, lines-depleted CySCs adopted several characteristics of hub cells, including the recruitment of new CySCs. This led us to examine the developmental relationship between CySCs and hub cells. In contrast to a previous report, we did not observe significant conversion of steady-state CySC progeny to hub fate. However, we found that these two cell types derive from a common precursor pool during gonadogenesis. Furthermore, lines mutant embryos exhibited gonads containing excess hub cells, indicating that lines represses hub cell fate during gonadogenesis. In many tissues, lines acts antagonistically to bowl, and we found that this is true for hub specification, establishing bowl as a positively acting factor in the development of the testis niche. |
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Authors:
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Stephen Dinardo; Tishina Okegbe; Lindsey Wingert; Sarah Freilich; Natalie Terry |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 138 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-13 Completed Date: 2011-07-01 Revised Date: 2012-09-18 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 1687-96 Citation Subset: IM |
Affiliation:
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Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine and the Penn Institute for Regenerative Medicine, Philadelphia, PA 19104-6048, USA. sdinardo@zimbra.upenn.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Body Patterning / genetics Carrier Proteins / genetics, physiology* Cell Dedifferentiation / genetics, physiology Cell Differentiation / genetics, physiology Cell Proliferation DNA-Binding Proteins / genetics, physiology* Drosophila / embryology*, genetics, metabolism Drosophila Proteins / genetics, physiology* Embryo, Nonmammalian Germ Cells / cytology, metabolism Male Stem Cell Niche* Testis / cytology*, embryology*, metabolism Transcription Factors / genetics, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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R0160804//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/DNA-Binding Proteins; 0/Drosophila Proteins; 0/Transcription Factors; 0/bowl protein, Drosophila; 0/lines protein, Drosophila |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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