Document Detail


On-line monitoring of the methanol concentration in Pichia pastoris cultures producing an heterologous lipase by sequential injection analysis.
MedLine Citation:
PMID:  14677700     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein was demonstrated. Linear response, observed up to 2 g l(-1), was reached by including a dilution chamber in the SIA manifold. The sampling frequency was 7 analyses per hour with a relative standard deviation lower than 4%.
Authors:
A Surribas; O Cos; J L Montesinos; F Valero
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Biotechnology letters     Volume:  25     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-12-17     Completed Date:  2004-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1795-800     Citation Subset:  IM    
Affiliation:
Departament d'Enginyeria Química, ETSE, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Bioreactors
Biosensing Techniques / instrumentation,  methods
Cell Culture Techniques / instrumentation*,  methods
Equipment Design
Equipment Failure Analysis / methods*
Flow Injection Analysis / instrumentation*,  methods
Lipase / biosynthesis*,  genetics
Methanol / analysis*,  metabolism*
Online Systems / instrumentation*
Pichia / genetics,  growth & development,  metabolism*
Protein Engineering / instrumentation
Recombinant Proteins / biosynthesis
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 67-56-1/Methanol; EC 3.1.1.3/Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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