| On-line microdialysis coupled with microbore liquid chromatography with ultraviolet detection for continuous monitoring of free cefsulodin in rat blood. | |
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MedLine Citation:
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PMID: 11358235 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A microdialysis method followed by a microbore liquid chromatographic ultraviolet detection procedure has been performed for the assay of unbound cefsulodin in rat blood. A microdialysis probe was inserted into the jugular vein for blood sampling. This method involves an on-line design for submitting dialysate into the liquid chromatographic system. The chromatographic conditions consisted of a mobile phase of methanol-100 mM monosodium phosphoric acid (10:90, v/v, pH 5.0) pumped through a microbore reversed-phase column at a flow-rate of 0.05 ml/min. Detection wavelength was set at 265 nm. Microdialysis probes, being laboratory-made, were screened for acceptable in vivo recovery while chromatographic resolution and detection were validated for response linearity as well as intra- and inter-day variabilities. The method was then applied to pharmacokinetics profiling of cefsulodin in the blood following intravenous administration of cefsulodin (20 mg/kg) in rats. Pharmacokinetics were calculated from the corrected data for dialysate concentrations of cefsulodin versus time. Based on pharmacokinetic calculation, cefsulodin best fitted to a two-exponential disposition. This study provided specific pharmacokinetic information for protein-unbound cefsulodin and demonstrated the applicability of this continuous sampling method for pharmacokinetic study. |
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Authors:
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T H Tsai; F C Cheng; Y F Chen; C F Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. A Volume: 914 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-05-18 Completed Date: 2001-09-13 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 83-7 Citation Subset: IM |
Affiliation:
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National Research Institute of Chinese Medicine, Shih-Pai, Taipei, Taiwan. thtsai@cma23.nricm.edu.tw |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cefsulodin / blood*, pharmacokinetics Chromatography, Liquid / methods* Male Microdialysis / methods* Rats Rats, Sprague-Dawley Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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62587-73-9/Cefsulodin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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