Document Detail


On-line microdialysis coupled with microbore liquid chromatography with ultraviolet detection for continuous monitoring of free cefsulodin in rat blood.
MedLine Citation:
PMID:  11358235     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A microdialysis method followed by a microbore liquid chromatographic ultraviolet detection procedure has been performed for the assay of unbound cefsulodin in rat blood. A microdialysis probe was inserted into the jugular vein for blood sampling. This method involves an on-line design for submitting dialysate into the liquid chromatographic system. The chromatographic conditions consisted of a mobile phase of methanol-100 mM monosodium phosphoric acid (10:90, v/v, pH 5.0) pumped through a microbore reversed-phase column at a flow-rate of 0.05 ml/min. Detection wavelength was set at 265 nm. Microdialysis probes, being laboratory-made, were screened for acceptable in vivo recovery while chromatographic resolution and detection were validated for response linearity as well as intra- and inter-day variabilities. The method was then applied to pharmacokinetics profiling of cefsulodin in the blood following intravenous administration of cefsulodin (20 mg/kg) in rats. Pharmacokinetics were calculated from the corrected data for dialysate concentrations of cefsulodin versus time. Based on pharmacokinetic calculation, cefsulodin best fitted to a two-exponential disposition. This study provided specific pharmacokinetic information for protein-unbound cefsulodin and demonstrated the applicability of this continuous sampling method for pharmacokinetic study.
Authors:
T H Tsai; F C Cheng; Y F Chen; C F Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  914     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-05-18     Completed Date:  2001-09-13     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  83-7     Citation Subset:  IM    
Affiliation:
National Research Institute of Chinese Medicine, Shih-Pai, Taipei, Taiwan. thtsai@cma23.nricm.edu.tw
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MeSH Terms
Descriptor/Qualifier:
Animals
Cefsulodin / blood*,  pharmacokinetics
Chromatography, Liquid / methods*
Male
Microdialysis / methods*
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
62587-73-9/Cefsulodin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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