| The limitations and validities of senescence associated-beta-galactosidase activity as an aging marker for human foreskin fibroblast Hs68 cells. | |
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MedLine Citation:
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PMID: 16154306 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The senescence associated-beta-galactosidase (SA-betaG) assay has become one of the most commonly used markers of cell-aging. However, the reliability of the assay is questionable because the enzyme is a non-specific marker for cell-aging. In this study, we found that the SA-betaG activity increased with cell age as well as in confluent quiescent cells or cells under serum starvation, and in cells treated with H2O2. Importantly, we found that SA-betaG activity was irreversibly increased in the senescent cells or H2O2-teated cells, but was reversible in quiescent cells induced by serum starvation or confluence. Using fluorescein di-beta-d-galactopyranoside (FDG) method for SA-betaG detection, we showed that senescent human foreskin fibroblast Hs68 cells did not express a specific enzyme that has a maximal activity at pH 6.0. In the pH profile of the cellular betaG activity in senescent Hs68 cells, only a single peak was found (with maximum at pH 4.6), and no addition peak was found at or around pH 6.0 that could be attributed to the SA-betaG activity. These results support the contention that SA-betaG is the lysosomal betaG that is detectable at suboptimal pH (i.e. pH 6.0) and demonstrate that cell-aging is not the only factor that can increase SA-betaG activity, rendering SA-betaG activity unspecific for cell-aging. Thus, the assay for cell-aging is only reliable when these confounding factors are controlled or excluded. |
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Authors:
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Nae-Cherng Yang; Miao-Lin Hu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-09-08 |
Journal Detail:
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Title: Experimental gerontology Volume: 40 ISSN: 0531-5565 ISO Abbreviation: Exp. Gerontol. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-10-10 Completed Date: 2006-04-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: England |
Other Details:
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Languages: eng Pagination: 813-9 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Chungchou Institute of Technology, Changhua, Taiwan, ROC. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aged Biological Markers / analysis Cell Aging / physiology* Cell Line Fibroblasts / enzymology* Histocytochemistry / methods Humans Male Models, Biological Skin beta-Galactosidase / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; EC 3.2.1.23/beta-Galactosidase |
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