Document Detail


The leukocyte protein L-plastin induces proliferation, invasion and loss of E-cadherin expression in colon cancer cells.
MedLine Citation:
PMID:  16287074     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
L-plastin, a gene that codes for an actin-bundling protein, is upregulated in the metastatic colon cancer cell line SW620, when compared to its premetastatic counterpart SW480. The aim of our study was to characterise the effect of L-plastin overexpression on SW480 cells in the context of the acquisition of a metastatic phenotype. SW480 cell lines overexpressing L-plastin were established (SW480-LPL). Analysis of these cell lines revealed significantly higher rates of proliferation and invasion than the control cell line (SW480-Ctrl). In addition, the expression of E-cadherin was lost from SW480-LPL cells. Treatment of SW480-LPL cells with cytochalasin B, an inhibitor of endocytosis, attenuated the loss of E-cadherin expression in these cells. The association of L-plastin overexpression with an increased rate of proliferation and invasion, and loss of E-cadherin expression in the SW480 colon cancer cell line indicates that L-plastin plays an important mechanistic role in colorectal cancer metastasis (supplementary material for this article can be found on the International Journal of Cancer website at http://www.interscience.wiley.com/jpages/0020-7136/suppmat/index.html).
Authors:
Eilis Foran; Peter McWilliam; Dermot Kelleher; David T Croke; Aideen Long
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  118     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-02-21     Completed Date:  2006-04-18     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2098-104     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2005 Wiley-Liss, Inc.
Affiliation:
Department of Biochemistry, Royal College of Surgeons in Ireland, Dublin 2, and Department of Clinical Medicine, Trinity College Dublin, St. James's Hospital, Dublin 8, Republic of Ireland.
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MeSH Terms
Descriptor/Qualifier:
Blood Proteins / biosynthesis*,  physiology
Cadherins / biosynthesis*
Cell Proliferation*
Colonic Neoplasms / genetics*,  pathology*
Gene Expression Profiling
Humans
Microfilament Proteins
Neoplasm Invasiveness
Neoplasm Metastasis*
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/Cadherins; 0/LCP1 protein, human; 0/Microfilament Proteins

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