Document Detail


The lens influences aqueous humor levels of transforming growth factor-beta 2.
MedLine Citation:
PMID:  9561366     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Transforming growth factor-beta 2 (TGF-beta 2) is a pluripotent cytokine which has been suggested to play a number of roles in ocular physiologic and pathologic states. Intraocular fluid (i.o.f.) levels of TGF-beta 2 are quite high. Although the sources of ocular TGF-beta are not completely defined, the retinal pigment epithelium, the epithelium of the ciliary body and trabecular meshwork cells all secrete it. In this study we utilized canine lens and rabbit ciliary pigmented epithelial cell cultures to quantitate the in vitro secretion of TGF-beta 2. In addition, the effects of aphakia or the presence of cataractous lenses on IOF TGF-beta 2 levels were determined. METHODS: Lens and ciliary body epithelial cell culture supernatants and aqueous humors were assayed for total TGF-beta 2 levels by ELISA and bioassay. RESULTS: TGF-beta 2 accumulated in the media bathing lens epithelial cell cultures (0.7 +/- 0.03 ng/ml at day 2) and ciliary pigmented epithelial cell cultures (0.8 +/- 0.06 ng/ml at day 2) in a time-dependent manner. Surprisingly, aqueous humor from aphakic rabbit eyes contained significantly higher levels of TGF-beta 2 than their contralateral phakic controls. Furthermore, aqueous humor from canine eyes with cataracts also contained significantly higher levels of TGF-beta 2 than normal eyes. CONCLUSIONS: These results suggest that the lens secretes TGF-beta 2 and that the presence and status of the lens may influence IOF TGF-beta 2 levels.
Authors:
J B Allen; M G Davidson; M P Nasisse; L N Fleisher; M C McGahan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie     Volume:  236     ISSN:  0721-832X     ISO Abbreviation:  Graefes Arch. Clin. Exp. Ophthalmol.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-06-08     Completed Date:  1998-06-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8205248     Medline TA:  Graefes Arch Clin Exp Ophthalmol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  305-11     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Physiology and Radiology, College of Veterinary Medicine, Raleigh, NC 27606, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aphakia / etiology,  metabolism,  pathology
Aqueous Humor / metabolism*
Biological Assay
Cataract / etiology,  metabolism,  pathology
Cells, Cultured
Ciliary Body / cytology,  metabolism
Disease Models, Animal
Dogs
Enzyme-Linked Immunosorbent Assay
Female
Lens, Crystalline / cytology,  metabolism*
Male
Pigment Epithelium of Eye / cytology,  metabolism
Rabbits
Transforming Growth Factor beta / secretion*
Grant Support
ID/Acronym/Agency:
EY05722/EY/NEI NIH HHS; EY08688/EY/NEI NIH HHS; EY11364/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Transforming Growth Factor beta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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