Document Detail

The leech receptor protein tyrosine phosphatase HmLAR2 is concentrated in growth cones and is involved in process outgrowth.
MedLine Citation:
PMID:  9477317     Owner:  NLM     Status:  MEDLINE    
Developing neurons extend long processes to specific distal targets using extracellular molecules as guidance cues to navigate through the embryo. Growth cones, specialized structures at the tip of the extending processes, are thought to accomplish this navigation through receptors that recognize guidance cues and modulate growth accordingly. In Drosophila, several receptor tyrosine phosphatases (rPTPs), including DLAR, have been shown to participate in directing neurite outgrowth. As yet, however, it is not known how rPTPs act to affect navigation. To gain insight into the mechanisms of rPTP-mediated outgrowth guidance, we have investigated the role of HmLAR2, a Hirudo medicinalis homologue of DLAR, in process outgrowth. HmLAR2 is expressed by, among other cells, a transient neuron-like template cell, the Comb cell. Here we present evidence that HmLAR2 protein becomes concentrated within their growth cones at a stage when C cell processes undergo rapid outgrowth. When antibodies raised against the extracellular domain of HmLAR2 were injected into intact embryos, they bound specifically to the C cell surface at growth cones and along processes and caused the partial internalization of HmLAR2 receptors. Moreover, the C cell processes were found to project aberrantly, to deviate from their normally highly regular trajectories and to extend shorter distances in the presence of the antibodies. We propose that HmLAR2 is required by the C cell for guidance and extension and suggest that it functions via its ectodomain to transduce extracellular guidance cues.
T R Gershon; M W Baker; M Nitabach; E R Macagno
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  125     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-05-04     Completed Date:  1998-05-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1183-90     Citation Subset:  IM    
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
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MeSH Terms
Antibodies / immunology,  pharmacology
Drosophila / growth & development
Leeches / embryology,  growth & development*
Muscle Development
Muscles / embryology
Nerve Tissue Proteins*
Neurites / metabolism*
Protein Tyrosine Phosphatases*
Receptor-Like Protein Tyrosine Phosphatases, Class 2
Receptors, Cell Surface / immunology,  metabolism*
Reg. No./Substance:
0/Antibodies; 0/Nerve Tissue Proteins; 0/Receptors, Cell Surface; EC Tyrosine Phosphatases; EC Protein Tyrosine Phosphatases, Class 2

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