Document Detail

A lamp light-emitting diode-induced fluorescence detector for capillary electrophoresis.
MedLine Citation:
PMID:  18585292     Owner:  NLM     Status:  MEDLINE    
A light-emitting diode-induced fluorescence detector (LED-FD) for capillary electrophoresis was constructed and evaluated. A lamp LED with an enhanced emission spectrum and a band pass filter was used as the excitation light source. Refractive index matching fluid (RIMF) was used in the detection cell to reduce scattering light and the noise level. The limit of detection (LOD) for fluorescein was 1.5 nM (SNR=3). The system exhibited linear responses in the range of 1 x 10(-8) to 5 x 10(-6)M (R=0.999). Application of the lamp LED-FD for the analysis of FITC-labeled ephedra herb extract by capillary electrophoresis was demonstrated.
Jing Xu; Yan Xiong; Shiheng Chen; Yafeng Guan
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-26
Journal Detail:
Title:  Talanta     Volume:  76     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-30     Completed Date:  2008-08-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  369-72     Citation Subset:  IM    
Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
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MeSH Terms
Electrophoresis, Capillary / instrumentation*
Fluorescein / analysis
Fluorescein-5-isothiocyanate / analysis
Reg. No./Substance:
2321-07-5/Fluorescein; 3326-32-7/Fluorescein-5-isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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