| lacZ as a genetic reporter for real-time MRI. | |
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MedLine Citation:
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PMID: 20146234 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Molecular imaging based on MRI is currently hampered by the lack of genetic reporters for in vivo imaging. We determined that the commercially available substrate S-Gal can be used to detect genetically engineered beta-galactosidase expressing cells by MRI. The effect and specificity of the reaction between beta-galactosidase and S-Gal on MRI contrast were determined both in vitro and in vivo. beta-galactosidase activity in the presence of S-Gal resulted in enhanced T(2) and T*(2) MR-contrast, which was amplified with increasing magnetic field strengths (4.7-17.6 T) in phantom studies. Using both lacZ(+) transgenic animals and lacZ(+) tissue transplants, we were able to detect labeled cells in live animals in real time. Similar to phantom studies, detection of the labeled cells/tissues in vivo was enhanced at high magnetic fields. These results demonstrate that the genetic reporter, lacZ, can be used as an in vivo marker gene using high-field-strength MRI. |
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Authors:
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Niclas E Bengtsson; Gary Brown; Edward W Scott; Glenn A Walter |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 63 ISSN: 1522-2594 ISO Abbreviation: Magn Reson Med Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-03 Completed Date: 2010-06-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: United States |
Other Details:
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Languages: eng Pagination: 745-53 Citation Subset: IM |
Copyright Information:
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(c) 2010 Wiley-Liss, Inc. |
Affiliation:
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Program in Stem Cell Biology and Regenerative Medicine, University of Florida, Gainesville, Florida 32610, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Marrow / physiology* Computer Systems Genes, Reporter / genetics Lac Operon / genetics* Magnetic Resonance Imaging / methods* Mice Mice, Inbred C57BL beta-Galactosidase / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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HL78670/HL/NHLBI NIH HHS; R01 HL7528/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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