Document Detail


A laboratory exercise to illustrate increased salivary cortisol in response to three stressful conditions using competitive ELISA.
MedLine Citation:
PMID:  17327591     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Perceived stress activates the hypothalamus-pituitary-adrenal axis, resulting in the release of glucocorticoids into the systemic circulation. Glucocorticoids cause the elevation of blood glucose, providing the necessary energy for the organism to cope with stress. Here, we outline a laboratory exercise that uses a competitive ELISA kit to illustrate the response of salivary cortisol concentrations to three stressful conditions. Twelve undergraduate students in the General and Comparative Endocrinology course at Iowa State University were subjected to presentation stress, fasting stress, and competition stress to determine their effect on salivary cortisol concentrations. Students had elevated salivary cortisol in response to each of these stresses compared with basal conditions. These results reiterate the importance of glucocorticoids as mediators of the stress response. This study also incorporates the use of the ELISA technique, a modern laboratory tool used to determine the amount of endogenous antigens in plasma or saliva. This laboratory exercise can easily be adapted to fit into already existing physiology and endocrinology curriculums.
Authors:
Mark F Haussmann; Carol M Vleck; Eugenia S Farrar
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Advances in physiology education     Volume:  31     ISSN:  1522-1229     ISO Abbreviation:  Adv Physiol Educ     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-28     Completed Date:  2007-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100913944     Medline TA:  Adv Physiol Educ     Country:  United States    
Other Details:
Languages:  eng     Pagination:  110-5     Citation Subset:  IM    
Affiliation:
Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa, USA. haussmannm@kenyon.edu
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MeSH Terms
Descriptor/Qualifier:
Adult
Competitive Behavior / physiology*
Enzyme-Linked Immunosorbent Assay
Fasting / physiology
Female
Humans
Hydrocortisone / analysis*
Iowa
Male
Physiology / education*
Research / education*
Saliva / chemistry*
Stress, Psychological / physiopathology*
Teaching / methods*
Chemical
Reg. No./Substance:
50-23-7/Hydrocortisone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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