| l-Amino acid oxidase-induced apoptosis in filamentous Botrytis cinerea. | |
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MedLine Citation:
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PMID: 21951783 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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As opposed to single-cell yeast and mammalian cell lines, apoptosis has not been greatly investigated in filamentous fungi because antibodies to the relevant fungal apoptosis-related proteins are not available commercially and because multicellular organisms cannot be studied using flow cytometry. Here we demonstrate how antibodies from a nonfungal source could be used to investigate this pathway. We show that apoptosis in the filamentous fungus Botrytis cinerea is triggered by the mitochondria-mediated caspase pathway, with release of the apoptotic factors cytochrome c, caspase 3, and caspase 9, on treatment with Trichoderma harzianum-derived l-amino acid oxidase. |
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Authors:
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Chi-Hua Cheng; Chia-Ann Yang; Shu-Ying Liu; Chaur-Tsuen Lo; Kou-Cheng Peng |
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Publication Detail:
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Type: - Date: 2011-9-7 |
Journal Detail:
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Title: Analytical biochemistry Volume: - ISSN: 1096-0309 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Institute of Biotechnology, National Dong-Hwa University, Hualien 97401, Taiwan, ROC. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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