Document Detail


A knob-associated tandem repeat in maize capable of forming fold-back DNA segments: are chromosome knobs megatransposons?
MedLine Citation:
PMID:  9724782     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A class of tandemly repeated DNA sequences (TR-1) of 350-bp unit length was isolated from the knob DNA of chromosome 9 of Zea mays L. Comparative fluorescence in situ hybridization revealed that TR-1 elements are also present in cytologically detectable knobs on other maize chromosomes in different proportions relative to the previously described 180-bp repeats. At least one knob on chromosome 4 is composed predominantly of the TR-1 repeat. In addition, several small clusters of the TR-1 and 180-bp repeats have been found in different chromosomes, some not located in obvious knob heterochromatin. Variation in restriction fragment fingerprints and copy number of the TR-1 elements was found among maize lines and among maize chromosomes. TR-1 tandem arrays up to 70 kilobases in length can be interspersed with stretches of 180-bp tandem repeat arrays. DNA sequence analysis and restriction mapping of one particular stretch of tandemly arranged TR-1 units indicate that these elements may be organized in the form of fold-back DNA segments. The TR-1 repeat shares two short segments of homology with the 180-bp repeat. The longest of these segments (31 bp; 64% identity) corresponds to the conserved region among 180-bp repeats. The polymorphism and complex structure of knob DNA suggest that, similar to the fold-back DNA-containing giant transposons in Drosophila, maize knob DNA may have some properties of transposable elements.
Authors:
E V Ananiev; R L Phillips; H W Rines
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  95     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-09-28     Completed Date:  1998-09-28     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10785-90     Citation Subset:  IM    
Affiliation:
Department of Agronomy and Plant Genetics and Plant Molecular Genetics Institute, University of Minnesota, 1991 Upper Buford Circle, St. Paul, MN 55108, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF071121;  AF071122;  AF071123;  AF071124;  AF071125;  AF071126;  AF071127
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosome Mapping*
DNA Transposable Elements*
DNA, Plant / genetics*
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Repetitive Sequences, Nucleic Acid*
Restriction Mapping
Zea mays / genetics*
Chemical
Reg. No./Substance:
0/DNA Transposable Elements; 0/DNA, Plant
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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