| A kinetoplastid-specific kinesin is required for cytokinesis and for maintenance of cell morphology in Trypanosoma brucei. | |
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MedLine Citation:
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PMID: 22168367 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Kinesins are motor-based transport proteins that play diverse roles in various cellular processes. The trypanosome genome lacks the homologues of many conserved mitotic kinesins, but encodes a number of trypanosome-specific kinesins with unknown function. Here, we report the biochemical and functional characterization of TbKIN-C, a trypanosome-specific kinesin, which was initially identified through an RNAi screen for cytokinesis genes in T. brucei. TbKIN-C possesses in vitro ATPase activity and associates with cytoskeletal tubulin microtubules in vivo. It is distributed throughout the cytoskeleton with a focal enrichment at the posterior end of the cell during early cell cycle stages. RNAi of TbKIN-C resulted in distorted cell shape with an elongated posterior filled with tyrosinated tubulin microtubules. Silencing of TbKIN-C impaired the segregation of organelles and cytoskeletal structures and led to detachment of the new flagellum and a small portion of the cytoplasm. We also show that RNAi of TbKIN-C compromised cytokinesis and abolished the trans-localization of TbCPC1, a subunit of the chromosomal passenger complex, from the central spindle to the initiation site of cytokinesis. Our results suggest an essential role of TbKIN-C in maintaining cell morphology, likely through regulating microtubule dynamics at the posterior end of the cell. |
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Authors:
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Liu Hu; Huiqing Hu; Ziyin Li |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-01-04 |
Journal Detail:
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Title: Molecular microbiology Volume: 83 ISSN: 1365-2958 ISO Abbreviation: Mol. Microbiol. Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-19 Completed Date: 2012-05-18 Revised Date: 2013-05-22 |
Medline Journal Info:
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Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 565-78 Citation Subset: IM |
Copyright Information:
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© 2011 Blackwell Publishing Ltd. |
Affiliation:
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Department of Microbiology & Molecular Genetics, University of Texas Medical School at Houston, TX 77030, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphatases
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genetics,
metabolism Cell Cycle Cytokinesis* Cytoskeleton / metabolism Flagella / metabolism Kinesin / genetics, metabolism* Microtubules / metabolism Mitochondrial Proteins / genetics, metabolism* Protozoan Proteins / genetics, metabolism* RNA Interference Trypanosoma brucei brucei / cytology*, genetics Tubulin / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AI090070/AI/NIAID NIH HHS; R56 AI090070/AI/NIAID NIH HHS; R56 AI090070-01A1/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Mitochondrial Proteins; 0/Protozoan Proteins; 0/Tubulin; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/Kinesin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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