Document Detail


The kinetics of interaction between lymphocytes and magnetic polymer particles.
MedLine Citation:
PMID:  2794518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Magnetic polymer-coated particles linked to antibodies are considered to be an efficient rosetting matrix for immunoselection. We have shown that a 20:1 bead:target cell ratio and a 90 min incubation period are the optimal conditions for specific binding of monoclonal antibody-labelled cells to goat anti-mouse IgG-coated beads. Higher ratios or longer incubation periods resulted in considerable non-specific binding. Characterisation of the optimal conditions for specific depletion of lymphocyte subpopulations showed that (a) a range of bead:target cell ratios and incubation periods can be used, with resulting high efficiency and specificity; (b) multiple monoclonal antibodies can be used simultaneously for the depletion of diverse lymphocyte subpopulations; (c) non-specific bead-to-cell binding does not affect the specificity and efficiency of magnetic depletion; (d) specific binding of one bead only was adequate for effective magnetic separation. These findings define the most economical, specific and efficient conditions of use of beads for negative immunoselection but preclude the use of beads as an analytical rosetting medium.
Authors:
D Pilling; G D Kitas; M Salmon; P A Bacon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  122     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1989-10-30     Completed Date:  1989-10-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  235-41     Citation Subset:  IM    
Affiliation:
Department of Rheumatology, University of Birmingham, Medical School, U.K.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / diagnostic use*
Cell Separation
Humans
Lymphocyte Depletion*
Lymphocytes / immunology*
Magnetics
Polymers
T-Lymphocytes / immunology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Polymers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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