Document Detail


The kinetics of the hypoferraemic response and changes in levels of alternative complement activity in diploid and triploid Atlantic salmon, following injection of lipopolysaccharide.
MedLine Citation:
PMID:  11417720     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To study any possible effects of triploidy on the kinetics of the response of two non-specific disease factors, full sibling diploid and triploid Atlantic salmon were injected intraperitoneally with either lipopolysaccharide (1 mg kg(-1) body weight) or saline. Individually marked fish were repetitively blood sampled for up to 19 days. Total serum protein concentrations remained constant throughout the experiment indicating that the sampling regime did not cause haemodilution. The alternative complement pathway activity (measured by the titre of haemolytic activity against rabbit erythrocytes) in the serum of saline injected fish remained constant but in LPS-injected fish it fell to barely detectable levels 2 days after injection, but recovered to pre-treatment levels by about day 5. Triploid fish took slightly longer to reach full recovery levels than diploids. All groups of fish showed a hypoferraemic response, suggesting that the sampling regime was at least partially responsible. However, the response was more rapid and pronounced in the LPS-injected fish. In the latter, serum iron concentrations decreased to very low levels by day 2 post-injection in the diploid fish and by day 3 in the triploid fish. Pre-treatment iron levels were re-established by about 15 days post-injection in all groups. The data show only slight differences between the diploid and triploid fish, but the longer time taken for the triploids to recover complement activity and the slower onset of the hypoferraemic response following injection of LPS, suggest that they may be at a disadvantage compared with their diploid siblings in their defence against bacterial infections.
Authors:
A L Langston; R Johnstone; A E Ellis
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Fish & shellfish immunology     Volume:  11     ISSN:  1050-4648     ISO Abbreviation:  Fish Shellfish Immunol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-06-21     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9505220     Medline TA:  Fish Shellfish Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  333-45     Citation Subset:  IM    
Affiliation:
Marine Laboratory, Aberdeen, Scotland, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Proteins / analysis
Complement Pathway, Alternative / drug effects*
Escherichia coli
Female
Injections, Intraperitoneal / veterinary
Iron / blood*,  metabolism
Kinetics
Lipopolysaccharides / administration & dosage,  pharmacology*
Male
Ploidies
Rabbits
Salmo salar / blood,  genetics,  immunology*
Transferrin / drug effects*,  metabolism
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/Lipopolysaccharides; 11096-37-0/Transferrin; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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