| A kinetic study of the growth of fatty acid vesicles. | |
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MedLine Citation:
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PMID: 15298905 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Membrane vesicles composed of fatty acids can be made to grow and divide under laboratory conditions, and thus provide a model system relevant to the emergence of cellular life. Fatty acid vesicles grow spontaneously when alkaline micelles are added to buffered vesicles. To investigate the mechanism of this process, we used stopped-flow kinetics to analyze the dilution of non-exchanging FRET probes incorporated into preformed vesicles during growth. Oleate vesicle growth occurs in two phases (fast and slow), indicating two pathways for the incorporation of fatty acid into preformed vesicles. We propose that the fast phase, which is stoichiometrically limited by the preformed vesicles, results from the formation of a "shell" of fatty acid around a vesicle, followed by rapid transfer of this fatty acid into the preformed vesicle. The slower phase may result from incorporation of fatty acid which had been trapped in an intermediate state. We provide independent evidence for the rapid transformation of micelles into an aggregated intermediate form after transfer from high to low pH. Our results show that the most efficient incorporation of added oleate into oleic acid/oleate vesicles occurs under conditions that avoid a large transient increase in the micelle/vesicle ratio. |
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Authors:
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Irene A Chen; Jack W Szostak |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biophysical journal Volume: 87 ISSN: 0006-3495 ISO Abbreviation: Biophys. J. Publication Date: 2004 Aug |
Date Detail:
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Created Date: 2004-08-09 Completed Date: 2005-02-17 Revised Date: 2010-09-21 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 988-98 Citation Subset: IM |
Affiliation:
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Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Colloids
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chemistry* Computer Simulation Fatty Acids / chemistry Hydrogen-Ion Concentration Kinetics Liposomes / chemistry* Membrane Fluidity* Micelles Models, Chemical* Oleic Acid / chemistry* Phosphatidylethanolamines / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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T32-GM07753/GM/NIGMS NIH HHS; T32-GM08313/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Colloids; 0/Fatty Acids; 0/Liposomes; 0/Micelles; 0/Phosphatidylethanolamines; 112-80-1/Oleic Acid; 3026-45-7/1,2-dipalmitoyl-3-phosphatidylethanolamine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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