Document Detail


A kinetic study of bovine haemoglobin hydrolysis by pepsin immobilized on a functionalized alumina to prepare hydrolysates containing bioactive peptides.
MedLine Citation:
PMID:  15032740     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hydrolysis kinetics of native and denatured haemoglobin, using pepsin immobilized on aluminium oxide, was studied in order to produce hydrolysates containing bioactive peptides. Pepsin was immobilized on acidic alumina and on 2-ethanolamine- O -phosphate (2-EAOP)-modified acidic alumina. Surface charge of the two supports was determined as a function of pH. Kinetic studies were performed at 23 degrees C in 0.1 M acetate buffer, pH 4.5. At this pH, the surface charge of the two supports was almost the same. The coating of alumina by 2-EAOP only introduced a two carbon spacer between alumina surface and the reaction medium. Adsorption on the two supports of haemoglobin, haem and peptides produced in the course of hydrolyses were compared. Fixation of 2-EAOP on a pepsin-alumina complex gave hydrolysis kinetics of urea-denatured haemoglobin close to that obtained with the same amount of pepsin in solution, but with comparatively less adsorption of peptides and complete adsorption of haem. Heterogeneous hydrolyses of haemoglobin with pepsin, immobilized on functionalized alumina, resulted in the presence of VV-haemorphin-4, VV-haemorphin-7 and neokyotorphin in the supernatants without haem, the presence of which makes further purification difficult.
Authors:
Elena-Loredana Ticu; Dominique Vercaigne-Marko; Anca Huma; Vlad Artenie; Ovidiu Toma; Didier Guillochon
Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  39     ISSN:  0885-4513     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-22     Completed Date:  2004-12-20     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  199-208     Citation Subset:  IM    
Affiliation:
Laboratorul de Biochimie, Facultatea de Biologie, Universitatea Al. I. Cuza, Iaşi, Romania.
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MeSH Terms
Descriptor/Qualifier:
Aluminum Oxide / chemistry*
Animals
Cattle
Coated Materials, Biocompatible / chemistry*
Enzyme Activation
Enzyme Stability
Enzymes, Immobilized / chemistry
Hemoglobins / chemistry*
Hydrogen-Ion Concentration
Hydrolysis
Kinetics
Materials Testing
Pepsin A / chemistry*
Peptides / chemical synthesis*
Protein Hydrolysates / chemical synthesis*
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Enzymes, Immobilized; 0/Hemoglobins; 0/Peptides; 0/Protein Hydrolysates; 1344-28-1/Aluminum Oxide; EC 3.4.23.1/Pepsin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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