Document Detail


A kinetic method amenable to automation for ceruloplasmin estimation with inexpensive and stable reagents.
MedLine Citation:
PMID:  17367772     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: To develop a kinetic, inexpensive, automatable assay for ceruloplasmin as ferroxidase. DESIGN AND METHODS: Dithiothreitol has been used to stabilize the substrate and quinolones to complex the ferric. RESULTS: Mean ferroxidase activity in healthy adults, Wilson's disease and pregnancy was 787.29, 178.5 and 1828.09 IU/L, respectively. Correlation coefficient of ferroxidase vis-à-vis copper, ferroxidase by ferrozine and ceruloplasmin were 0.90, 0.94 and 0.92, respectively. CONCLUSIONS: Norfloxacin method is a simple, inexpensive and automatable kinetic assay.
Authors:
Babu Lal Somani; Vivek Ambade
Publication Detail:
Type:  Journal Article     Date:  2007-02-08
Journal Detail:
Title:  Clinical biochemistry     Volume:  40     ISSN:  0009-9120     ISO Abbreviation:  Clin. Biochem.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-23     Completed Date:  2007-07-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  571-4     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Armed Forces Medical College, Pune-411040, India. blsomani@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Ceruloplasmin / analysis*,  metabolism*
Clinical Chemistry Tests / economics,  methods*
Dithiothreitol / chemistry
Female
Ferrozine / chemistry
Hepatolenticular Degeneration / enzymology
Humans
Hydrogen-Ion Concentration
Kinetics
Male
Norfloxacin / chemistry
Pregnancy
Quinolones / chemistry
Reproducibility of Results
Chemical
Reg. No./Substance:
0/Quinolones; 28048-33-1/Ferrozine; 3483-12-3/Dithiothreitol; 70458-96-7/Norfloxacin; EC 1.16.3.1/Ceruloplasmin

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