Document Detail


Ste20/GCK kinase Nak1/Orb3 polarizes the actin cytoskeleton in fission yeast during the cell cycle.
MedLine Citation:
PMID:  15731009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polar growth is a crucial process during cell morphogenesis. The microtubule and actin cytoskeletons, and vesicular transport are tightly regulated to direct cellular growth and to generate specific cell forms. We demonstrate here that the Ste20-related protein kinase Nak1/Orb3 is required in fission yeast to polarize the actin cytoskeleton at the tips of the cells and for cell separation, and so is involved in controlling both cell shape and late stages of cytokinesis. The localization of the Nak1/Orb3 kinase to the cell tips, a medial ring and the spindle-pole bodies changes during the cell cycle, and the accumulation of F-actin at the cell tips is dependent on Nak1/Orb3 kinase. The phosphorylation of Nak1/Orb3 is periodic during the cell cycle and could be part of a mechanism that relocalizes a constitutively active kinase from the cell tips to the middle of the cell, thereby coordinating reorganization of the actin cytoskeleton and regulation of cell separation with cell-cycle progression.
Authors:
Klaus Leonhard; Paul Nurse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  118     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-25     Completed Date:  2005-07-26     Revised Date:  2012-06-08    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1033-44     Citation Subset:  IM    
Affiliation:
Cancer Research UK, Cell Cycle Laboratory, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK. leonhak@rockefeller.edu
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Actomyosin / metabolism
Carrier Proteins / physiology*
Cell Cycle
Cell Proliferation
Cloning, Molecular
Cytokinesis
Cytoskeleton / metabolism*
DNA, Complementary / metabolism
Gene Deletion
Gene Expression Regulation, Fungal*
Genotype
Green Fluorescent Proteins / metabolism
Intracellular Signaling Peptides and Proteins
Microscopy, Fluorescence
Mutation
Phenotype
Phosphoric Monoester Hydrolases / metabolism
Phosphorylation
Protein Binding
Protein-Serine-Threonine Kinases / metabolism*
Proto-Oncogene Proteins c-myc / metabolism
Saccharomyces cerevisiae Proteins / metabolism*
Schizosaccharomyces / metabolism*
Schizosaccharomyces pombe Proteins / physiology*
Temperature
Time Factors
Chemical
Reg. No./Substance:
0/Actins; 0/Carrier Proteins; 0/DNA, Complementary; 0/Intracellular Signaling Peptides and Proteins; 0/Nak1 protein, S pombe; 0/Proto-Oncogene Proteins c-myc; 0/Saccharomyces cerevisiae Proteins; 0/Schizosaccharomyces pombe Proteins; 147336-22-9/Green Fluorescent Proteins; 9013-26-7/Actomyosin; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.25/STE20 protein, S cerevisiae; EC 3.1.3.-/Phosphoric Monoester Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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