| The kinase activity of aurora B is required for kinetochore-microtubule interactions during mitosis. | |
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MedLine Citation:
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PMID: 12062052 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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As a component of the "chromosomal passenger protein complex," the aurora B kinase is associated with centromeres during prometaphase and with midzone microtubules during anaphase and is required for both mitosis and cytokinesis. Ablation of aurora B causes defects in both prometaphase chromosomal congression and the spindle checkpoint; however, the mechanisms underlying these defects are unclear. To address this question, we have examined chromosomal movement, spindle organization, and microtubule motor distribution in NRK cells transfected with a kinase-inactive, dominant-negative mutant of aurora B, aurora B(K-R). In cells overexpressing aurora B(K-R) fused with GFP, centromeres moved in a synchronized and predominantly unidirectional manner, as opposed to the independent, bidirectional movement in control cells expressing a similar level of wild-type aurora B-GFP. In addition, most kinetochores became physically separated from spindle microtubules, which appeared as a striking bundle between the spindle poles. These defects were associated with a microtubule-dependent depletion of motor proteins dynein and CENP-E from kinetochores. Our observations suggest that aurora B regulates the association of motor proteins with kinetochores during prometaphase. Interactions of kinetochore motors with microtubules may in turn regulate the organization of microtubules, the movement of prometaphase chromosomes, and the release of the spindle checkpoint. |
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Authors:
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Maki Murata-Hori; Yu-li Wang |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Current biology : CB Volume: 12 ISSN: 0960-9822 ISO Abbreviation: Curr. Biol. Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-06-13 Completed Date: 2002-12-09 Revised Date: 2011-07-11 |
Medline Journal Info:
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Nlm Unique ID: 9107782 Medline TA: Curr Biol Country: England |
Other Details:
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Languages: eng Pagination: 894-9 Citation Subset: IM |
Affiliation:
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Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01605, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Fluorescent Antibody Technique Green Fluorescent Proteins Kinetochores / physiology* Luminescent Proteins / metabolism Microinjections Microtubules / physiology* Mitosis / physiology* Protein-Serine-Threonine Kinases / metabolism*, physiology Rats Recombinant Fusion Proteins / metabolism Transfection |
| Grant Support | |
ID/Acronym/Agency:
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GM 32476/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Luminescent Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/aurora kinase |
| Comments/Corrections | |
Comment In:
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Curr Biol. 2002 Jul 9;12(13):R458-60
[PMID:
12121637
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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