| A keratin K5Cre transgenic line appropriate for tissue-specific or generalized Cre-mediated recombination. | |
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MedLine Citation:
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PMID: 15124227 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe here a mouse line bearing a bovine keratin K5Cre recombinase transgene. These mice showed a dual pattern of Cre-mediated recombination, depending on the parent transmitting the transgene. In paternal transmission, recombination occurred specifically in the skin and stratified epithelia-as expected according to the expression of endogenous keratin K5. However, constitutive recombination between loxP sites transmitted by the sperm took place when the mother possessed the K5Cre transgene, even when the transgene was absent in the progeny. Cre expression in late-stage oocytes, with the Cre protein persisting into the developing embryo, leads to the constitutive recombination observed. Thus, this transgenic line allows for both tissue-specific and generalized recombination, depending on the breeding scheme. |
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Authors:
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Angel Ramirez; Angustias Page; Alberto Gandarillas; Jennifer Zanet; Sophie Pibre; Miguel Vidal; Laura Tusell; Anna Genesca; Duncan A Whitaker; David W Melton; Jose L Jorcano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Genesis (New York, N.Y. : 2000) Volume: 39 ISSN: 1526-954X ISO Abbreviation: Genesis Publication Date: 2004 May |
Date Detail:
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Created Date: 2004-05-04 Completed Date: 2005-03-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100931242 Medline TA: Genesis Country: United States |
Other Details:
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Languages: eng Pagination: 52-7 Citation Subset: IM |
Copyright Information:
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Copyright 2004 Wiley-Liss, Inc. |
Affiliation:
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Epithelial Damage, Repair and Tissue Engineering Program, CIEMAT, Madrid, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Blotting, Northern DNA Primers Fluorescent Antibody Technique Genotype Keratins / genetics, physiology* Mice Mice, Transgenic Recombinases / metabolism* Recombination, Genetic* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Recombinases; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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