Document Detail


A karyometric note on nucleoli in human early granulocytic precursors.
MedLine Citation:
PMID:  17007106     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The diameter of nucleoli was measured in human bone marrow early granulocytic precursors after visualization by a simple cytochemical method for demonstration of RNA. Such method facilitated to clearly see nucleolar bodies without perinucleolar chromatin, including those of micronucleoli. The bone marrow of patients suffering from chronic myeloid leukaemia (untreated with cytostatics) provided a satisfactory number of both myeloblasts and promyelocytes for nucleolar measurements because of prevailing granulopoiesis. The direct nucleolar measurement was carried out on digitized and processed images on the screen at magnification 4,300x. It seems to be likely that the nucleolar size is directly related to the number of nucleoli per cell. The largest nucleoli were present in both myeloblasts and promyelocytes that possessed a single nucleolus. In contrast, the nucleolar diameter was significantly smaller in cells with multiple nucleoli. However, in cells with small multiple nucleoli, one of them was always larger and dominant with a large number of AgNORs. Such large nucleoli are possibly visible in specimens stained with panoptic procedures or methods staining nuclear chromatin or DNA. It should also be mentioned that both myeloblasts and promyelocytes mostly possessed two nucleoli with the mean diameter close to 1.5 microm. The incidence of early granulocytic precursors classified according to the nucleolar number and size strongly suggested that the various nucleolar number and nucleolar size in these cells might be related to the different stage of the cell cycle and might also explain their heterogeneity.
Authors:
K Smetana; D Mikulenková; I Jirásková; H Klamová
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Folia biologica     Volume:  52     ISSN:  0015-5500     ISO Abbreviation:  Folia Biol. (Praha)     Publication Date:  2006  
Date Detail:
Created Date:  2006-09-29     Completed Date:  2007-02-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0234640     Medline TA:  Folia Biol (Praha)     Country:  Czech Republic    
Other Details:
Languages:  eng     Pagination:  16-20     Citation Subset:  IM    
Affiliation:
Clinical Department, Institute of Haematology and Blood Transfusion, Prague, Czech Republic. karel.smetana@uhkt.cz
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleolus / ultrastructure*
Granulocyte Precursor Cells / ultrastructure*
Histocytochemistry
Humans
Karyometry
Nucleolus Organizer Region / ultrastructure
RNA / analysis
Chemical
Reg. No./Substance:
63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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