Document Detail


The isolation and in vitro expansion of hepatic Sca-1 progenitor cells.
MedLine Citation:
PMID:  19233130     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The intra-hepatic population of liver progenitor cells expands during liver injury when hepatocyte proliferation is inhibited. These cells can be purified by density gradient centrifugation and cultured. Separated by size only this population contains small cells of hematopoietic, epithelial and endothelial lineages and is thought to contain liver stem cells. The identity of liver stem cells remains unknown although there is some evidence that tissue Sca1(+) CD45(-) cells display progenitor cell characteristics. We identified both intra-hepatic and gall bladder Sca1(+) cells following liver injury and expanded ex vivo Sca1 cells as part of heterogenous cell culture or as a purified population. We found significant difference between the proliferation of Sca-1 cells when plated on laminin or collagen I while proliferation of heterogenous population was not affected by the extracellular matrix indicating the necessity for culture of Sca1(+) cells with laminin matrix or laminin producing cells in long term liver progenitor cell cultures.
Authors:
Elizabeth Clayton; Stuart J Forbes
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-20
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  381     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-30     Completed Date:  2009-04-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  549-53     Citation Subset:  IM    
Affiliation:
MRC/Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, UK. Elizabeth.Clayton@ed.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Ly / analysis*
Cell Lineage
Cell Proliferation
Cell Separation
Liver / chemistry,  cytology*
Membrane Proteins / analysis*
Mice
Mice, Inbred C57BL
Stem Cells / chemistry,  cytology*
Grant Support
ID/Acronym/Agency:
//Medical Research Council
Chemical
Reg. No./Substance:
0/Antigens, Ly; 0/Ly6a protein, mouse; 0/Membrane Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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