Document Detail


The isolation and comparison of cellulase genes from two strains of Ruminococcus albus.
MedLine Citation:
PMID:  2600588     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endo-1,4-beta-glucanase genes have been cloned from two strains of Ruminococcus albus recently isolated in this laboratory. Although the strains were phenotypically similar, cross-hybridization studies between them showed significant genetic differences, with only 20% of the genome forming DNA heteroduplexes. Heteroduplexes displayed an average dissociation temperature 9 degrees C lower than that of the homoduplex. Consistent with this, restriction maps of the two endoglucanase genes showed no similarity, and hybridization work using the endoglucanase genes as probes revealed that neither gene was present in the genome of the other isolate of R. albus. Comparative enzyme characterization showed differences between the enzymes in their response to temperature, pH and substrate preference.
Authors:
C E Ware; T Bauchop; K Gregg
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of general microbiology     Volume:  135     ISSN:  0022-1287     ISO Abbreviation:  J. Gen. Microbiol.     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1990-01-26     Completed Date:  1990-01-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375371     Medline TA:  J Gen Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  921-30     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Microbiology and Nutrition, University of New England, Armidale, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteria / classification,  enzymology,  genetics*
Blotting, Southern
Cellulase / genetics*
Cloning, Molecular / methods
Genes, Bacterial*
Restriction Mapping
Rumen / microbiology*
Sheep
Chemical
Reg. No./Substance:
EC 3.2.1.4/Cellulase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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