| The isolation and characters of L-forms and reversions of Bacillus licheniformis var. Endoparasiticus (Benedek) associated with the erythrocytes of clinically normal persons. | |
| | |
MedLine Citation:
|
PMID: 682179 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Thirty-eight strains of the Gram-positive bacterium identified as Bacillus licheniformis var. endoparasiticus (Benedek), referred to as BLE, were isolated in various stages of reversion form the L-forms, from 28 out of 100 samples of whole blood or erythrocytes from normal healthy subjects, after prolonged incubation. Similar results were obtained from 100 samples from hospital patients with conditions not usually associated with blood infection. BLE was isolated from only one of 125 samples of plasma, including those separated from infected erythrocytes. Isolates from cultures incubated for up to 4 months were usually in the form of spheroplasts or diphtheroid bacilli; the fully reverted phase, resembling B. licheniformis, with the capacity to form endospores, was isolated occasionally from cultures aged 1--6 months, and it constituted about half the isolates recovered from cultures aged 6--25 months. BLE was isolated in subculture, and with the usual frequency, in previously unopened, primary cultures. It did not occur in 1200 subcultures of 150 control cultures made with autoclaved or irradiated blood cells; it was not detected in the environment of the laboratory or blood-sampling areas, or on the skin or in the respiratory passages of the operators and other persons associated with the laboratory, where typical, saprophytic B. licheniformis was very rare. It is concluded that this Bacillus species exists as an L-form, associated with the erythrocytes of a large proportion of normal persons, as previously recorded by several observers. Some of the morphological variants associated with the L-cycles have in the past been described as different organisms, for example L-forms of various bacteria or mycoplasmas, and the diphtheroid stage has been thought to belong to the genera Corynebacterium and Listeria. The sporogenous stage, although frequently described, has normally been discounted as a contaminant. These observations do not admit of any conclusion in respect of the claims that such bacteria may have a role in arthritis, cancer or other diseases. |
| | |
Authors:
|
K A Bisset; R Bartlett |
Related Documents
:
|
12493169 - Use of simulated blood cultures for time to detection comparison between bact/alert and... 12823759 - Monitoring of apheresis platelet bacterial contamination with an automated liquid cultu... 11469499 - Rapid immunodiagnosis of streptococci and enterococci in blood cultures. 9090549 - High frequency of pseudobacteremia at a university hospital. 19348119 - Effectiveness of introducing blood culture collection packs to reduce contamination rates. 15615059 - A rapid microtechnique for in vitro stimulation of canine lymphocytes using whole blood. 7190579 - Legionnaires' disease bacterium (legionella pneumophila) multiples intracellularly in h... 12635669 - Does identification of the causal organism of corneal ulcers influence the outcome? 16535179 - Comparison of biomass dry weights and radial growth rates of fungal colonies on media s... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of medical microbiology Volume: 11 ISSN: 0022-2615 ISO Abbreviation: J. Med. Microbiol. Publication Date: 1978 Aug |
Date Detail:
|
Created Date: 1978-10-27 Completed Date: 1978-10-27 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0224131 Medline TA: J Med Microbiol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 335-49 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacillus
/
cytology,
isolation & purification*,
metabolism Erythrocytes / microbiology* Humans L Forms / isolation & purification*, metabolism Spheroplasts / metabolism |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of bursectomy and thymectomy on Pasteurella multocida infection in chickens.
Next Document: The routine application of a microtechnique for the demonstration of leptospiral antibodies.