Document Detail

The isolation and characterization of the soluble and membrane-bound porcine cytochrome b5 cDNAs.
MedLine Citation:
PMID:  9367886     Owner:  NLM     Status:  MEDLINE    
In some male pigs, there is an increased production of the testicular 16 androstene steroids which end up being concentrated in fatty tissue. When the meat is cooked, a disagreeable odor/flavor is produced, a phenomenon known as "boar taint." All boars selected for food production are castrated even though only ca 10% of boars may be "tainted." This has a high economic cost because castrated pigs convert food into meat less efficiently, the meat is fatter, and there is an increased mortality due to the castration procedure. Recent data has implicated an increased level of cytochrome b5 in the testes with the increased synthesis of the 16-androstene steroids. As an initial step in analyzing this process, we used 5' and 3' RACE PCR procedures to isolate, clone and sequence the cDNAs for the membrane-bound and soluble forms of porcine cytochrome b5.
P K VanDerMark; A W Steggles
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  240     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-12-15     Completed Date:  1997-12-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  80-3     Citation Subset:  IM    
Department of Biochemistry and Molecular Pathology, Northeastern Ohio Universities College of Medicine, Rootstown 44272, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF016388;  AF016389
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MeSH Terms
Amino Acid Sequence
Base Sequence
Blotting, Southern
Cytochromes b5 / blood,  chemistry*,  genetics,  isolation & purification*
DNA, Complementary / blood,  chemistry*,  isolation & purification*
Membrane Proteins / chemistry*,  genetics,  isolation & purification*
Molecular Sequence Data
Testis / enzymology
Reg. No./Substance:
0/DNA, Complementary; 0/Membrane Proteins; 9035-39-6/Cytochromes b5

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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