Document Detail


The isolation and characterization of the soluble and membrane-bound porcine cytochrome b5 cDNAs.
MedLine Citation:
PMID:  9367886     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In some male pigs, there is an increased production of the testicular 16 androstene steroids which end up being concentrated in fatty tissue. When the meat is cooked, a disagreeable odor/flavor is produced, a phenomenon known as "boar taint." All boars selected for food production are castrated even though only ca 10% of boars may be "tainted." This has a high economic cost because castrated pigs convert food into meat less efficiently, the meat is fatter, and there is an increased mortality due to the castration procedure. Recent data has implicated an increased level of cytochrome b5 in the testes with the increased synthesis of the 16-androstene steroids. As an initial step in analyzing this process, we used 5' and 3' RACE PCR procedures to isolate, clone and sequence the cDNAs for the membrane-bound and soluble forms of porcine cytochrome b5.
Authors:
P K VanDerMark; A W Steggles
Related Documents :
20003746 - Chicken salad as the source of a case of listeria monocytogenes infection in connecticut.
20573456 - Evaluation of hygiene practices and microbiological quality of cooked meat products dur...
12597486 - Assessment of control measures to achieve a food safety objective of less than 100 cfu ...
10549426 - Unusual food-borne pathogens. listeria monocytogenes, aeromonas, plesiomonas, and edwar...
6853936 - Vitamin contents of pre-prepared foods sampled from a hospital food service line.
18655236 - Personal exposure to fine particles and benzo[a]pyrene. relation with indoor and outdoo...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  240     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-12-15     Completed Date:  1997-12-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  80-3     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Pathology, Northeastern Ohio Universities College of Medicine, Rootstown 44272, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF016388;  AF016389
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Southern
Cytochromes b5 / blood,  chemistry*,  genetics,  isolation & purification*
DNA, Complementary / blood,  chemistry*,  isolation & purification*
Male
Membrane Proteins / chemistry*,  genetics,  isolation & purification*
Molecular Sequence Data
Solubility
Swine
Testis / enzymology
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Membrane Proteins; 9035-39-6/Cytochromes b5

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterization of the [NiFe] hydrogenase from the sulfate reducer Desulfovibrio vulgaris Hildenbor...
Next Document:  Upregulation of the anti-apoptotic protein Bcl-2 may be an early event in neurodegeneration: studies...